Activity
(Specific Activity: Sample activity data. for lot-specific data, please refer to the lot-specific datasheet.)
TtCsm6 recombinant protein
TtCsm6
Synonyms
TtCsm6; CRISPR system endoribronuclease Csm6; CRISPR type III-A associated protein Csm6; csm6; TtCsm6 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.
Tag
HIS
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample activity data. for lot-specific data, please refer to the lot-specific datasheet.)
Activity
(Specific Activity: Sample activity data. for lot-specific data, please refer to the lot-specific datasheet.)
SDS-Page
(Purity: Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.)
SDS-Page
(Purity: Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.)
Related Product Information for TtCsm6 recombinant protein
Recombinant Thermus thermophilus TtCsm6 was expressed in E Coli cells using a C -terminal His tag. The protein accession number is WP_011229148.
TtCsm6 is a single-stranded RNA-specific endoribonuclease protein that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by the HEPN domain dimer and it contributes to the Type-III-A CRISPR-Cas system (1). Upon activation of the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA non-specifically. TtCsm6 is activated by the cyclic oligoadenylate second messengers that was converted from ATP by the Cas10 subunit of the Type III interference complex (2).
TtCsm6 is a single-stranded RNA-specific endoribonuclease protein that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by the HEPN domain dimer and it contributes to the Type-III-A CRISPR-Cas system (1). Upon activation of the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA non-specifically. TtCsm6 is activated by the cyclic oligoadenylate second messengers that was converted from ATP by the Cas10 subunit of the Type III interference complex (2).
Product Categories/Family for TtCsm6 recombinant protein
References
1. Niewoehner, O. et al: Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. Rna 22(3):318-329,2016. 2. Niewoehner, O. et al: Type III CRISPR-Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548, 2017.
NCBI and Uniprot Product Information
Molecular Weight
53kDa
Similar Products
Product Notes
The TtCsm6 (Catalog #AAA516767) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TtCsm6, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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