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Early B-Cell Factor 1 Recombinant Protein | EBF1 recombinant protein
Recombinant Early B-Cell Factor 1 (EBF1)
Gene Names
EBF1; EBF; COE1; OLF1; O/E-1
Reactivity
Human
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Early B-Cell Factor 1; Recombinant Early B-Cell Factor 1 (EBF1); EBF1 recombinant protein
Host
E.coli
Reactivity
Human
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose and Proclin300.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Applicable Applications for EBF1 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Asp179~Ser451
Tags
N-terminal His Tag
Subcellular Location
Nucleus
Traits
Freeze-dried powder
Predicted isoelectric point
9.3
Predicted Molecular Mass
33.9kDa
Accurate Molecular Mass
34/17kDa as determined by SDS-PAGE reducing conditions
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not
vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Early B-cell factor 1
NCBI Official Synonym Full Names
early B-cell factor 1
NCBI Official Symbol
EBF1
NCBI Official Synonym Symbols
EBF; COE1; OLF1; O/E-1
NCBI Protein Information
transcription factor COE1
UniProt Protein Name
Transcription factor COE1
Protein Family
UniProt Gene Name
EBF1
UniProt Synonym Gene Names
COE1; EBF; O/E-1; OE-1
UniProt Entry Name
COE1_HUMAN
Uniprot Description
EBF1: Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Belongs to the COE family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription regulation
Chromosomal Location of Human Ortholog: 5q34
Cellular Component: nucleus
Molecular Function: protein dimerization activity; DNA binding; metal ion binding; transcription factor activity
Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; multicellular organismal development
Research Articles on EBF1
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Product Notes
The EBF1 ebf1 (Catalog #AAA2031633) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Early B-Cell Factor 1 (EBF1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Early B-Cell Factor 1 can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, WB (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the EBF1 ebf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Early B-Cell Factor 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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