Human azurocidin 1 ELISA Kit | AZU1 elisa kit

Human Azurocidin/Heparin Binding Protein, AZU/HBP ELISA Kit

Cat. Num. AAA702182

• Gene Names: AZU1; AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
• Reactivity: Human
• Synonyms: azurocidin 1; Human Azurocidin/Heparin Binding Protein; AZU/HBP ELISA Kit; Human Azurocidin/Heparin Binding Protein (AZU/HBP) ELISA Kit; AZAMP; AZU; CAP37; HBP; HUMAZUR; NAZC; cationic antimicrobial protein 37; heparin-binding protein; neutrophil azurocidin; AZU1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat AVP. No significant cross-reactivity or interference between rat AVP and analogues was observed.
• Sequence Length: 251

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: 0.078 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AZU1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AVP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AVP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AVP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AVP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 11342670
• NCBI GeneID: 566
• NCBI Accession #: NP_001691.1
• NCBI GenBank Nucleotide #: NM_001700.3
• UniProt Accession #: P20160; P80014; Q52LG4; Q9UCM1; Q9UCT5
• Molecular Weight: 26,886 Da
• NCBI Official Full Name: azurocidin preproprotein
• NCBI Official Synonym Full Names: azurocidin 1
• NCBI Official Symbol: AZU1
• NCBI Official Synonym Symbols: AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
• NCBI Protein Information: azurocidin; neutrophil azurocidin; heparin-binding protein; cationic antimicrobial protein 37; cationic antimicrobial protein CAP37
• UniProt Protein Name: Azurocidin
• Protein Family: Azurocidin
• UniProt Gene Name: AZU1
• UniProt Synonym Gene Names: HBP
• UniProt Entry Name: CAP7_HUMAN

NCBI Description

Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. The protein encoded by this gene is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.2 PublicationsManual assertion based on experiment in:Ref.10