Human Trypsin-2 (PRSS2) ELISA Kit | TRY2 elisa kit

Human Trypsin-2 (PRSS2) ELISA Kit

Cat. Num. AAA9714775

• Gene Names: PRSS2; TRY2; TRY8; TRYP2
• Reactivity: Human
• Synonyms: Trypsin-2 (PRSS2); Human Trypsin-2 (PRSS2) ELISA Kit; PRSS2; MGC111183; MGC120174; TRY8; TRYP2; anionic trypsinogen; protease serine 2 preproprotein; protease; serine; 2; preproprotein; trypsin 2; trypsin; trypsinogen 2; TRY2 elisa kit

• Reactivity: Human
• Specificity: Human Trypsin-2 (PRSS2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS2. No significant cross-reactivity or interference between Human PRSS2 and analogues was observed.
• Sequence Length: 65

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Trypsin-2 (PRSS2) Standard Curve.)

Related Product Information for TRY2 elisa kit

Description: This Human Trypsin-2 (PRSS2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS2 in samples. An antibody specific for PRSS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRSS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: In the ileum, PRSS2 may be involved in defensin processing, including DEFA5. PRSS2 is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins.Rowen et al. (1996) mapped the gene corresponding to the third pancreatic trypsinogen cDNA by fluorescence in situ hybridization. They used a cosmid clone containing 3 trypsinogen genes. Strong hybridization to chromosome 7 and weaker hybridization to chromosome 9 were observed. They isolated and partially sequenced 4 cosmid clones from the chromosome 9 region. They found that the region represents a duplication and translocation of a DNA segment from the 3-prime end of the TCRB locus that includes at least 7 V(beta) elements and a functional trypsinogen gene denoted T9.

NCBI and Uniprot Product Information

• NCBI GI #: 119572308
• NCBI GeneID: 5645
• UniProt Accession #: P07478
• NCBI Official Full Name: protease, serine, 2 (trypsin 2), isoform CRA_b
• NCBI Official Synonym Full Names: serine protease 2
• NCBI Official Symbol: PRSS2
• NCBI Official Synonym Symbols: TRY2; TRY8; TRYP2
• NCBI Protein Information: trypsin-2
• UniProt Protein Name: Trypsin-2
• Protein Family: Transcriptional regulator of yeast form adherence
• UniProt Gene Name: PRSS2
• UniProt Synonym Gene Names: TRY2; TRYP2
• UniProt Entry Name: TRY2_HUMAN

NCBI Description

This gene belongs to the trypsin family of serine proteases and encodes anionic trypsinogen. It is part of a cluster of trypsinogen genes that are located within the T cell receptor beta locus. Enzymes of this family cleave peptide bonds that follow lysine or arginine residues. This protein is found at high levels in pancreatic juice and its upregulation is a characteristic feature of pancreatitis. This protein has also been found to activate pro-urokinase in ovarian tumors, suggesting a function in tumor invasion. In addition, this enzyme is able to cleave across the type II collagen triple helix in rheumatoid arthritis synovitis tissue, potentially participating in the degradation of type II collagen-rich cartilage matrix. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2015]

Uniprot Description

trypsin 2: In the ileum, may be involved in defensin processing, including DEFA5. Belongs to the peptidase S1 family.

Protein type: Secreted; Extracellular matrix; Secreted, signal peptide; Calcium-binding; EC 3.4.21.4; Protease; Cell adhesion

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; digestion; innate immune response; proteolysis; positive regulation of cell growth

Disease: Pancreatitis, Hereditary