Rat tripartite motif-containing 72 ELISA Kit | TRIM72 elisa kit

Rat Tripartite motif-containing protein 72, TRIM72 ELISA Kit

Cat. Num. AAA901204

• Gene Names: TRIM72; MG53
• Reactivity: Rat
• Synonyms: tripartite motif-containing 72; Rat Tripartite motif-containing protein 72; TRIM72 ELISA Kit; Rat Tripartite motif-containing protein 72 (TRIM72) ELISA kit; MG53; mitsugumin-53; TRIM72 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat TRIM72. No significant cross-reactivity or interference between rat TRIM72 and analogues was observed.
• Sequence Length: 477

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 18.75 pg/ml -1200 pg/ml.
• Sensitivity: The minimum detectable dose of rat TRIM72 is typically less than 4.7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRIM72 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRIM72 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRIM72 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIM72 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIM72 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126723064
• NCBI GeneID: 100008639
• NCBI Accession #: NP_001075484.1
• NCBI GenBank Nucleotide #: NM_001082015.1
• UniProt Accession #: Q1XH18
• Molecular Weight: 52,779 Da
• NCBI Official Full Name: tripartite motif-containing protein 72
• NCBI Official Symbol: TRIM72
• NCBI Official Synonym Symbols: MG53
• NCBI Protein Information: tripartite motif-containing protein 72; mitsugumin 53; mitsugumin-53
• UniProt Protein Name: Tripartite motif-containing protein 72
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM72
• UniProt Synonym Gene Names: MG53; Mg53
• UniProt Entry Name: TRI72_RABIT

Uniprot Description

Function: Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca2+-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles