Human neurogranin (protein kinase C substrate, RC3) ELISA Kit | NRGN elisa kit

Human Neurogranin, NRGN ELISA Kit

Cat. Num. AAA940053

• Gene Names: NRGN; RC3; hng
• Reactivity: Human
• Synonyms: neurogranin (protein kinase C substrate; RC3); Human Neurogranin; NRGN ELISA Kit; Human Neurogranin (NRGN) ELISA kit; RC3; hng; calmodulin-binding protein; neurogranin; protein kinase C substrate; NRGN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human NRGN. No significant cross-reactivity or interference between human NRGN and analogues was observed.
• Sequence Length: 78

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 18.75 ng/ml -1200 ng/ml.
• Sensitivity: The minimum detectable dose of human NRGN is typically less than 4.68 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRGN elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRGN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRGN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 187131239
• NCBI GeneID: 4900
• NCBI Accession #: NP_001119653.1
• NCBI GenBank Nucleotide #: NM_001126181.1
• UniProt Accession #: Q92686
• Molecular Weight: 7,618 Da
• NCBI Official Full Name: neurogranin
• NCBI Official Synonym Full Names: neurogranin (protein kinase C substrate, RC3)
• NCBI Official Symbol: NRGN
• NCBI Official Synonym Symbols: RC3; hng
• NCBI Protein Information: neurogranin; ng; calmodulin-binding protein; protein kinase C substrate
• UniProt Protein Name: Neurogranin
• Protein Family: Neurogranin
• UniProt Gene Name: NRGN
• UniProt Synonym Gene Names: Ng
• UniProt Entry Name: NEUG_HUMAN

NCBI Description

Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. [provided by RefSeq, Jul 2008]

Uniprot Description

neurogranin: Acts as a third messenger substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium. Belongs to the neurogranin family.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 11q24

Cellular Component: intracellular

Molecular Function: calmodulin binding

Biological Process: nervous system development; signal transduction