SDS-Page
TRDMT1 recombinant protein
TRDMT1, 1-391aa, Human, His tag, E Coli
Gene Names
TRDMT1; DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
TRDMT1; 1-391aa; Human; His tag; E Coli; tRNA (cytosine (38)-C (5))-methyltransferase; M.HsaIIP; PuMet; RNMT1; DNMT2; TRDMT1 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMEPLRV LELYSGVGGM HHALRESCIP AQVVAAIDVN TVANEVYKYN FPHTQLLAKT IEGITLEEFD RLSFDMILMS PPCQPFTRIG RQGDMTDSRT NSFLYILDIL PRLQKLPKYI LLENVKGFEV SSTRDLLIQT IENCGFQYQE FLLSPTSLGI PNSRLRYFLI AKLQSEPLPF QAPGQVLMEF PKIESVHPQK YAMDVENKIQ EKNVEPNISF DGSIQCSGKD AILFKLETAE EIHRKNQQDS DLSVKMLKDF LEDDTDVNQY LLPPKSLLRY ALLLDIVQPT CRRSVCFTKG YGSYIEGTGS VLQTAEDVQV ENIYKSLTNL SQEEQITKLL ILKLRYFTPK EIANLLGFPP EFGFPEKITV KQRYRLLGNS LNVHVVAKLI KILYE
Sequence Length
391
Applicable Applications for TRDMT1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for TRDMT1 recombinant protein
TRDMT1 (tRNA aspartic acid methyltransferase 1), also known as DNMT2, specifically methylates cytosine 38 in the anticodon loop of tRNA (Asp). Recombinant human TRDMT1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for TRDMT1 recombinant protein
References
Okano M., et al. (1998) Nucleic Acids Res. 26:2536-2540; Franchina M., et al. (2001) Int. J. Biochem. Cell Biol. 33:1104-1115
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
47.2 kDa (415aa) confirmed by MALDI-TOF
NCBI Official Full Name
TRNA aspartic acid methyltransferase 1
NCBI Official Synonym Full Names
tRNA aspartic acid methyltransferase 1
NCBI Official Symbol
TRDMT1
NCBI Official Synonym Symbols
DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
NCBI Protein Information
tRNA (cytosine(38)-C(5))-methyltransferase
UniProt Protein Name
tRNA (cytosine(38)-C(5))-methyltransferase
Protein Family
UniProt Gene Name
TRDMT1
UniProt Synonym Gene Names
DNMT2; Dnmt2; DNA MTase homolog HsaIIP; M.HsaIIP
NCBI Description
This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]
Uniprot Description
Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
Research Articles on TRDMT1
Similar Products
Product Notes
The TRDMT1 trdmt1 (Catalog #AAA204429) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's TRDMT1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the TRDMT1 trdmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMEPLRV LELYSGVGGM HHALRESCIP AQVVAAIDVN TVANEVYKYN FPHTQLLAKT IEGITLEEFD RLSFDMILMS PPCQPFTRIG RQGDMTDSRT NSFLYILDIL PRLQKLPKYI LLENVKGFEV SSTRDLLIQT IENCGFQYQE FLLSPTSLGI PNSRLRYFLI AKLQSEPLPF QAPGQVLMEF PKIESVHPQK YAMDVENKIQ EKNVEPNISF DGSIQCSGKD AILFKLETAE EIHRKNQQDS DLSVKMLKDF LEDDTDVNQY LLPPKSLLRY ALLLDIVQPT CRRSVCFTKG YGSYIEGTGS VLQTAEDVQV ENIYKSLTNL SQEEQITKLL ILKLRYFTPK EIANLLGFPP EFGFPEKITV KQRYRLLGNS LNVHVVAKLI KILYE. It is sometimes possible for the material contained within the vial of "TRDMT1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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