Rabbit anti-Human PGA5 Polyclonal Antibody | anti-PGA5 antibody

PGA5, CT (PGA3,Pepsin A,PGA4,PGA5) (Biotin)

Cat. Num. AAA6332938

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PGA5; Polyclonal Antibody; CT (PGA3; Pepsin A; PGA4; PGA5) (Biotin); PGA3; anti-PGA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PGA5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PGA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-383 amino acids from the C-terminal region of human PGA5.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PGA5 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PGA5 antibody detected the PGA5 protein (arrow).)

Related Product Information for anti-PGA5 antibody

PGA3 shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Product Categories/Family for anti-PGA5 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Plebani, M., et al. Helicobacter 2(4):172-175(1997). Fujinaga, M., et al. Protein Sci. 4(5):960-972(1995) Zwiers, A., et al. Clin. Nephrol. 41(3):153-158(1994) Ichinose, M., et al. Jpn. J. Cancer Res. 82(6):686-692(1991) Athauda, S.B., et al. J. Biochem. 106(5):920-927(1989).

NCBI and Uniprot Product Information

• NCBI GI #: 23943854
• NCBI GeneID: 5222
• NCBI Accession #: NP_055039.1; NP_001073276.1
• NCBI GenBank Nucleotide #: NM_014224.3
• UniProt Accession #: P00790; Q7M4R0; Q8N1E3; A8K749; B7ZW62; B7ZW75; P0DJD9
• Molecular Weight: 41,993 Da
• NCBI Official Full Name: pepsin A-5 preproprotein
• NCBI Official Synonym Full Names: pepsinogen 5, group I (pepsinogen A)
• NCBI Official Symbol: PGA5
• NCBI Protein Information: pepsin A-5; pepsinogen-5; pepsinogen A5
• Protein Family: Pepsin

NCBI Description

This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]

Uniprot Description

PGA5: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Belongs to the peptidase A1 family.

Protein type: Secreted; EC 3.4.23.1; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Molecular Function: aspartic-type endopeptidase activity

Biological Process: digestion; proteolysis