SDS-PAGE
TRDMT1 (DNMT2) recombinant protein
TRDMT1 (DNMT2) recombinant protein
Gene Names
TRDMT1; DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
Applications
Western Blot
Synonyms
TRDMT1 (DNMT2); TRDMT1 (DNMT2) recombinant protein; DNMT2; DMNT2; PUMET; RNMT1; MHSAIIP
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Length
1361
Applicable Applications for TRDMT1 (DNMT2) recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human TRDMT1 (DNMT2) was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for TRDMT1 (DNMT2) recombinant protein
Recombinant full-length human TRDMT1 (DNMT2) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: TRDMT1 is responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. TRDMT1 also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, TRDMT1 is distinct and highly conserved in its function among various species. The indirect sequence recognition is also a feature of eukaryotic DNA methyltransferases, which may have arisen from a DNMT2-like RNA methyltransferase (1). DNMT2 is involved in retrotransposon silencing and telomere integrity in somatic cells (2).
Scientific Background: TRDMT1 is responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. TRDMT1 also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, TRDMT1 is distinct and highly conserved in its function among various species. The indirect sequence recognition is also a feature of eukaryotic DNA methyltransferases, which may have arisen from a DNMT2-like RNA methyltransferase (1). DNMT2 is involved in retrotransposon silencing and telomere integrity in somatic cells (2).
Product Categories/Family for TRDMT1 (DNMT2) recombinant protein
References
1. Goll, M. G. et.al: Methylation of tRNA(Asp) by the DNA methyltransferase homolog Dnmt2. Science 311: 395-397, 2006.
2. Phalke, S. et.al: Retrotransposon silencing and telomere integrity in somatic cells of Drosophila depends on the cytosine-5 methyltransferase DNMT2. Nature Genet. 41: 696-702, 2009.
2. Phalke, S. et.al: Retrotransposon silencing and telomere integrity in somatic cells of Drosophila depends on the cytosine-5 methyltransferase DNMT2. Nature Genet. 41: 696-702, 2009.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~67 kDa
NCBI Official Full Name
Homo sapiens tRNA aspartic acid methyltransferase 1, mRNA
NCBI Official Synonym Full Names
tRNA aspartic acid methyltransferase 1
NCBI Official Symbol
TRDMT1
NCBI Official Synonym Symbols
DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
NCBI Protein Information
tRNA (cytosine(38)-C(5))-methyltransferase; tRNA (cytosine(38)-C(5))-methyltransferase; DNA methyltransferase-2; DNA MTase homolog HsaIIP; tRNA (cytosine-5-)-methyltransferase; DNA (cytosine-5)-methyltransferase-like protein 2
UniProt Protein Name
tRNA (cytosine(38)-C(5))-methyltransferase
UniProt Gene Name
TRDMT1
UniProt Synonym Gene Names
DNMT2; Dnmt2; DNA MTase homolog HsaIIP; M.HsaIIP
UniProt Entry Name
TRDMT_HUMAN
NCBI Description
This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]
Uniprot Description
Function: Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). Ref.9
Catalytic activity: S-adenosyl-L-methionine + cytosine38 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA. Ref.9
Subcellular location: Nucleus
Probable.
Tissue specificity: Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes.
Sequence similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.Contains 1 SAM-dependent MTase C5-type domain.
Research Articles on TRDMT1 (DNMT2)
Similar Products
Product Notes
The TRDMT1 (DNMT2) trdmt1 (Catalog #AAA515267) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human TRDMT1 (DNMT2) was expressed by baculovirus in Sf9 insect cells. AAA Biotech's TRDMT1 (DNMT2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TRDMT1 (DNMT2) trdmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRDMT1 (DNMT2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
