Porcine Tryptase ELISA Kit | TPS elisa kit

Porcine Tryptase ELISA Kit

Cat. Num. AAA38309

• Gene Names: TPSAB1; TPS1; TPS2; TPSB1
• Reactivity: Porcine
• Synonyms: Tryptase; Porcine Tryptase ELISA Kit; TPS elisa kit

• Reactivity: Porcine
• Specificity: No significant cross-reactivity or interference between Porcine TPS and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.5 ng/ml - 16 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated TPS concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine TPS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TPS and TPS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TPS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for TPS elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated TPS concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine TPS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TPS and TPS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TPS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1182067
• NCBI GeneID: 7177
• UniProt Accession #: Q15661; P15157; Q15663; Q6B052; Q9H2Y4; Q9H2Y5; Q9UQI1; D2E6R9; D2E6S1
• Molecular Weight: 30,515 Da
• NCBI Official Full Name: tryptase
• NCBI Official Synonym Full Names: tryptase alpha/beta 1
• NCBI Official Symbol: TPSAB1
• NCBI Official Synonym Symbols: TPS1; TPS2; TPSB1
• NCBI Protein Information: tryptase alpha/beta-1; tryptase I; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase alpha-1; tryptase alpha II; mast cell beta I tryptase; mast cell alpha II tryptase
• UniProt Protein Name: Tryptase alpha/beta-1
• Protein Family: Tryptase
• UniProt Gene Name: TPSAB1
• UniProt Synonym Gene Names: TPS1; TPS2; TPSB1; Tryptase-1
• UniProt Entry Name: TRYB1_HUMAN

NCBI Description

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.21.59

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response; proteolysis