Mouse DHFR (Dihydrofolate reductase) ELISA Kit | DHFR elisa kit

Mouse DHFR (Dihydrofolate reductase) ELISA Kit

Cat. Num. AAA7606560

• Gene Names: Dhfr; Dhfr1
• Reactivity: Mouse
• Synonyms: DHFR (Dihydrofolate reductase); Mouse DHFR (Dihydrofolate reductase) ELISA Kit; DHFR; DHFRP1; DYR; dihydrofolate reductase; EC 1.5.1.3; DHFR elisa kit

• Reactivity: Mouse
• Sequence Length: 187

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DHFR elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DHFR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-DHFR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DHFR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DHFR can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 18426814
• NCBI GeneID: 24312
• NCBI Accession #: NP_569084.1
• NCBI GenBank Nucleotide #: NM_130400.2
• UniProt Accession #: Q920D2
• Molecular Weight: 21,638 Da
• NCBI Official Full Name: dihydrofolate reductase
• NCBI Official Synonym Full Names: dihydrofolate reductase
• NCBI Official Symbol: Dhfr
• NCBI Official Synonym Symbols: Dhfr1
• NCBI Protein Information: dihydrofolate reductase
• UniProt Protein Name: Dihydrofolate reductase
• Protein Family: Viral dihydrofolate reductase
• UniProt Gene Name: Dhfr

NCBI Description

key component of purine and pyrmidine biosynthesis pathway; NADPH-dependent molecule reduces dihydrofolate to tetrahydrofolate [RGD, Feb 2006]

Uniprot Description

DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family.

Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 1.5.1.3; Oxidoreductase

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: cytoplasm; mitochondrion

Molecular Function: dihydrofolate reductase activity; dihydrofolic acid binding; drug binding; folic acid binding; methotrexate binding; mRNA binding; NADP binding; translation repressor activity, nucleic acid binding

Biological Process: axon regeneration; dihydrofolate metabolic process; folic acid metabolic process; negative regulation of translation; positive regulation of nitric-oxide synthase activity; response to methotrexate; response to nicotine; tetrahydrobiopterin biosynthetic process; tetrahydrofolate biosynthetic process; tetrahydrofolate metabolic process