Thiopurine S-methyltransferase Recombinant Protein | TPMT recombinant protein

Recombinant Human Thiopurine S-methyltransferase

Cat. Num. AAA9421529

• Gene Names: TPMT; TPMTD
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Thiopurine S-methyltransferase; Recombinant Human Thiopurine S-methyltransferase; Thiopurine methyltransferase; TPMT recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Partial of the Full Length of 1-245aa, 4-244aa

• Tag Info: GST-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for TPMT recombinant protein

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.

NCBI and Uniprot Product Information

• NCBI GI #: 4507653
• NCBI GeneID: 7172
• NCBI Accession #: NP_000358.1
• NCBI GenBank Nucleotide #: NM_000367.4
• UniProt Accession #: P51580; O14806; O15423; O15424; O15425; O15426; O15515; O15548; O43213; Q5VUK6; Q9UBE6; Q9UBT8
• Molecular Weight: 55.1kD
• NCBI Official Full Name: thiopurine S-methyltransferase isoform 1
• NCBI Official Synonym Full Names: thiopurine S-methyltransferase
• NCBI Official Symbol: TPMT
• NCBI Official Synonym Symbols: TPMTD
• NCBI Protein Information: thiopurine S-methyltransferase
• UniProt Protein Name: Thiopurine S-methyltransferase
• Protein Family: Thiopurine S-methyltransferase
• UniProt Gene Name: TPMT

NCBI Description

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]

Uniprot Description

TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family.

Protein type: EC 2.1.1.67; Methyltransferase; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytosol

Molecular Function: thiopurine S-methyltransferase activity

Biological Process: methylation; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

Disease: Thiopurine S-methyltransferase Deficiency