Human Sp100 Nuclear Antigen ELISA Kit | Sp100 elisa kit

Human Sp100 Nuclear Antigen ELISA Kit

Cat. Num. AAA728608

• Gene Names: SP100; lysp100b
• Reactivity: Human
• Synonyms: Sp100 Nuclear Antigen; Human Sp100 Nuclear Antigen ELISA Kit; Sp100 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This Sp100 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Sp100. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Sp100 elisa kit

Principle of the Assay: Sp100 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Sp100. Standards or samples are then added to the microtiter plate wells and Sp100 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Sp100 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for Sp100 are added to each well to "sandwich" the Sp100 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Sp100 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Sp100 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Sp100 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 1255929
• NCBI GeneID: 6672
• UniProt Accession #: P23497; O75450; Q13343; Q8TE34; Q96F70; Q96T24; Q96T95; B4DDX5; B8ZZD8; E7EUA7; E9PH61; F8WFE2
• Molecular Weight: 50,032 Da
• NCBI Official Full Name: Sp100, partial
• NCBI Official Synonym Full Names: SP100 nuclear antigen
• NCBI Official Symbol: SP100
• NCBI Official Synonym Symbols: lysp100b
• NCBI Protein Information: nuclear autoantigen Sp-100; speckled 100 kDa; SP100-HMG nuclear autoantigen; nuclear dot-associated Sp100 protein
• UniProt Protein Name: Nuclear autoantigen Sp-100
• Protein Family: Nuclear autoantigen
• UniProt Gene Name: SP100
• UniProt Entry Name: SP100_HUMAN

NCBI Description

This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011]

Uniprot Description

SP100: May play a role in the control of gene expression. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; PML body; Mre11 complex; cytoplasm; nucleolus; nucleus

Molecular Function: protein domain specific binding; identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription coactivator activity; chromatin binding; kinase binding; transcription corepressor activity; transcription factor binding

Biological Process: retinoic acid receptor signaling pathway; negative regulation of protein export from nucleus; response to retinoic acid; viral reproduction; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of cell motility; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; chromatin remodeling; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; negative regulation of viral transcription; response to cytokine stimulus; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; telomere maintenance