Rabbit anti-Mouse, Rat MeCP2 Polyclonal Antibody | anti-MeCP2 antibody

MeCP2 (Methyl CpG Binding Protein 2 (Rett Syndrome), AUTSX3, MRX16, MRX79, PPMX, RTS, RTT) (MaxLight 405)

Cat. Num. AAA6487254

• Reactivity: Mouse, Rat
• Applications: Immunoblot, Western Blot, Western Blot
• Purity: Purified by Protein A Affinity Chromatography
• Synonyms: MeCP2; Polyclonal Antibody; MeCP2 (Methyl CpG Binding Protein 2 (Rett Syndrome); AUTSX3; MRX16; MRX79; PPMX; RTS; RTT) (MaxLight 405); anti-MeCP2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MeCP2, Mr 75kD. A protein at ~75kD was also observed in HeLa nuclear extract. This protein was competed by pre-incubation of the antibody with the immunogenic peptide, suggesting that some HeLa strains contain MeCP2 or a related protein, contrary to a previous report. Species Crossreactivity: Mouse and rat. Predicted to crossreact with human based on the conservation of the immunogenic sequence.
• Purity/Purification: Purified by Protein A Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-MeCP2 antibody: Immunoblot (IB), Western Blot (WB)
• Application Notes: IB: 0.5-2ug/ml of this lot detected MeCP2 in mouse heart nuclear extracts and a previous lot detected MeCP2 in mouse brain nuclear extracts and L6 nuclear extracts.; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated, synthetic peptide corresponding to aa465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Mouse heart nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MeCP2 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MeCP2 (~75kD).)

Product Categories/Family for anti-MeCP2 antibody

Antibodies; Disease Markers

References

1. Craig, J. M., et al., Hum Mol Genet. 12: 3109-21, 2003. 2. Bakker, J., et al., J. Biol. Chem. 277: 22573-80, 2002. 3. Guy, J., et al., Nat. Genet. 27: 322-6, 2001. 4. Nguyen, C. T., et al., Nucleic Acids Res. 29: 4598-606, 2001. 5. Yu, F., et al., Nucleic Acids Res. 29: 4493-501, 2001. 6. Bird, A.P. and A.P. Wolfe, Cell 99: 451-454, 1999.