Tissue Plasminogen Activator Active Protein | tPA active protein

Recombinant Human Tissue Plasminogen Activator

Cat. Num. AAA142404

• Gene Names: PLAT; TPA; T-PA
• Purity: Greater than 98.0% as determined by: ; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Synonyms: Tissue Plasminogen Activator; Recombinant Human Tissue Plasminogen Activator; Tissue-type plasminogen activator; EC 3.4.21.68; tPA; t-PA; t-plasminogen activator; TPA; T-PA; DKFZp686I03148.; tPA active protein

• Purity/Purification: Greater than 98.0% as determined by: ; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Form/Format: Each mg of t-PA contains 1.7 gr L-arginine, 0.5 gr phosphoric acid and 4 mg tween 80.
• Sequence Length: 562

• Source: Chinese Hamster Ovary Cells (CHO)
• Enzymatic Activity: 580,000 IU/mg.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized t-PA in sterile 18MΩ-cm H₂O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized t-PA although stable at room temperature, should be stored desiccated below -18°C.; Upon reconstitution tPA should be stored at 4°C between 2-7 days and for future use below -18°C.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Please prevent freeze-thaw cycles.

Related Product Information for tPA active protein

Tissue Plasminogen Activator Human Recombinant produced in CHO cells is a single, glycosylated polypeptide chain containing 527 amino acids and having a molecular mass of 59008.71 Dalton. tPA is a serine protease enzyme that converts plasminogen to plasmin. The tPA is purified by proprietary chromatographic techniques.

Product Categories/Family for tPA active protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4505861
• NCBI GeneID: 5327
• NCBI Accession #: NP_000921.1
• NCBI GenBank Nucleotide #: NM_000930.3
• UniProt Accession #: P00750; Q15103; Q503B0; Q6PJA5; Q7Z7N2; Q86YK8; Q9BU99; Q9BZW1; A8K022; B2R8E8
• NCBI Official Full Name: tissue-type plasminogen activator isoform 1 preproprotein
• NCBI Official Synonym Full Names: plasminogen activator, tissue
• NCBI Official Symbol: PLAT
• NCBI Official Synonym Symbols: TPA; T-PA
• NCBI Protein Information: tissue-type plasminogen activator; alteplase; plasminogen activator, tissue type; reteplase; t-plasminogen activator; tissue plasminogen activator (t-PA)
• UniProt Protein Name: Tissue-type plasminogen activator
• Protein Family: Taurine--pyruvate aminotransferase
• UniProt Gene Name: PLAT
• UniProt Synonym Gene Names: t-PA; t-plasminogen activator; tPA
• UniProt Entry Name: TPA_HUMAN

NCBI Description

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Vesicle; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; EC 3.4.21.68; Protease

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: negative regulation of proteolysis; synaptic transmission, glutamatergic; response to peptide hormone stimulus; fibrinolysis; response to cAMP; smooth muscle cell migration; response to hypoxia; regulation of synaptic plasticity; protein modification process; platelet-derived growth factor receptor signaling pathway; proteolysis; blood coagulation; transmembrane transport; plasminogen activation

Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator