Rat Transforming Growth Factor Alpha ELISA Kit | TGF-alpha elisa kit

Rat Transforming Growth Factor Alpha ELISA Kit

Cat. Num. AAA765046

• Gene Names: TGFA; TFGA
• Reactivity: Rat
• Synonyms: Transforming Growth Factor Alpha; Rat Transforming Growth Factor Alpha ELISA Kit; TGF-alpha/TGFalpha/TGFA/protransforming growth factor alpha/TFGA/transforming growth factor; alpha/transforming growth factor-alpha; TGF-alpha elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of TGF-alpha. No significant cross-reactivity or interference between TGF-alpha and analogues was observed.
• Sequence Length: 13

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for TGF-alpha elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 553791
• NCBI GeneID: 7039
• Molecular Weight: 17,329 Da
• NCBI Official Full Name: transforming growth factor alpha, partial
• NCBI Official Synonym Full Names: transforming growth factor alpha
• NCBI Official Symbol: TGFA
• NCBI Official Synonym Symbols: TFGA
• NCBI Protein Information: protransforming growth factor alpha
• UniProt Protein Name: Protransforming growth factor alpha
• Protein Family: Transforming growth factor
• UniProt Gene Name: TGFA
• UniProt Synonym Gene Names: TGF-alpha; ETGF
• UniProt Entry Name: TGFA_HUMAN

NCBI Description

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: basolateral plasma membrane; cell surface; cytoplasmic vesicle; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; extracellular space; Golgi membrane; integral to plasma membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: epidermal growth factor receptor binding; growth factor activity; protein binding

Biological Process: activation of MAPK activity; cell proliferation; cellular protein metabolic process; COPII coating of Golgi vesicle; epidermal growth factor receptor signaling pathway; ER to Golgi vesicle-mediated transport; positive regulation of epidermal growth factor receptor activity; positive regulation of epithelial cell proliferation; positive regulation of mitosis; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine