Mouse HCN1 Monoclonal Antibody | anti-Hcn1 antibody

HCN1 Antibody: PerCP

Cat. Num. AAA800458

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: HCN1; Monoclonal Antibody; HCN1 Antibody: PerCP; BCNG-1; BCNG1; Brain cyclic nucleotide gated channel 1; Brain cyclic nucleotide-gated channel 1; HAC2; HCN1_HUMAN; Hyperpolarization activated cyclic nucleotide gated potassium channel 1; anti-Hcn1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S70-28
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 910

• Applicable Applications for anti-Hcn1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Conjugate: PerCP
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.)

Western Blot (WB)

(Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28. Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

Related Product Information for anti-Hcn1 antibody

Background Info: Detects ~100kDa. No cross-reactivity against HCN2

Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, play a crucial role in the regulatons of cell excitability. Importantly, they contribute to spontaneous rhythmic activity in both the heart and brain (3).

Product Categories/Family for anti-Hcn1 antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232.

NCBI and Uniprot Product Information

• NCBI GI #: 16758108
• NCBI GeneID: 84390
• NCBI Accession #: NP_445827.1
• NCBI GenBank Nucleotide #: NM_053375.1
• UniProt Accession #: Q9JKBO; Q9JKB0
• Molecular Weight: 102,422 Da
• NCBI Official Full Name: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
• NCBI Official Synonym Full Names: hyperpolarization-activated cyclic nucleotide-gated potassium channel 1
• NCBI Official Symbol: Hcn1
• NCBI Protein Information: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
• UniProt Protein Name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
• Protein Family: Anaphase-promoting complex
• UniProt Gene Name: Hcn1
• UniProt Entry Name: HCN1_RAT

NCBI Description

may generate neuronal pacemaker activity [RGD, Feb 2006]

Uniprot Description

HCN1: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. Belongs to the potassium channel HCN family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass