Topoisomerase I, DNA Recombinant Protein
Topoisomerase I, DNA, Recombinant, Human (Scl-70)
Purity
Purified80% (SDS-PAGE)
Synonyms
Topoisomerase I; DNA; Recombinant; Human (Scl-70); DNA recombinant protein
Host
Sf9 insect cells
Purity/Purification
Purified
80% (SDS-PAGE)
80% (SDS-PAGE)
Form/Format
Supplied as a liquid in 16mM HEPES, pH 8.0, 250mM sodium chloride and 20% glycerol.
Application Notes
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Preparation and Storage
-20 degree C
Stability: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Please avoid freeze-thaw cycles.
Stability: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Please avoid freeze-thaw cycles.
Related Product Information for Topoisomerase I, DNA recombinant protein
DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis. In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100 kD molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kD molecular weight from which the Scl-70 antigen has derived its name. Recombinant Human Scl-70 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 dalton. Recombinant Scl-70 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Product Categories/Family for Topoisomerase I, DNA recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91.58kD
NCBI Official Full Name
topoisomerase I
Uniprot Description
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
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Product Notes
The Topoisomerase I, DNA (Catalog #AAA637514) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera. Researchers should empirically determine the suitability of the Topoisomerase I, DNA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Topoisomerase I, DNA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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