Rabbit anti-Human NFYB Polyclonal Antibody | anti-NFYB antibody

Rabbit Polyclonal to Human NFYB

Cat. Num. AAA240980

• Gene Names: NFYB; HAP3; CBF-A; CBF-B; NF-YB
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Delipidated and defibrinated
• Synonyms: NFYB; Polyclonal Antibody; Rabbit Polyclonal to Human NFYB; Anti-NFYB Antibody (N-Terminus) IHC-plus; CBF-A; CBF-B; HAP3; NF-YB; Human NFYB; anti-NFYB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Purity/Purification: Delipidated and defibrinated
• Form/Format: 0.01% sodium azide.
• Concentration: 90 mg/ml (varies by lot)
• Sequence Length: 207

• Applicable Applications for anti-NFYB antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA, IHC-P (1:500), IP, WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Antigen Modification: N-Terminus
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)

NCBI and Uniprot Product Information

• NCBI GI #: 5453780
• NCBI GeneID: 4801
• NCBI Accession #: NP_006157.1
• NCBI GenBank Nucleotide #: NM_006166.3
• UniProt Accession #: P25208; Q96IY8; A8K7B9
• Molecular Weight: 22,831 Da
• NCBI Official Full Name: nuclear transcription factor Y subunit beta
• NCBI Official Synonym Full Names: nuclear transcription factor Y, beta
• NCBI Official Symbol: NFYB
• NCBI Official Synonym Symbols: HAP3; CBF-A; CBF-B; NF-YB
• NCBI Protein Information: nuclear transcription factor Y subunit beta; Transcription factor NF-Y, B subunit; CAAT box DNA-binding protein subunit B; nuclear transcription factor Y subunit B; CCAAT-binding transcription factor subunit A
• UniProt Protein Name: Nuclear transcription factor Y subunit beta
• Protein Family: Nuclear transcription factor
• UniProt Gene Name: NFYB
• UniProt Synonym Gene Names: HAP3; NF-YB
• UniProt Entry Name: NFYB_HUMAN

NCBI Description

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]

Uniprot Description

NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; protein complex binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cellular lipid metabolic process