Typical Testing Data/Standard Curve (for reference only)
Human Dipeptidase 3 (DPEP3) ELISA Kit | DPEP3 elisa kit
Human Dipeptidase 3 (DPEP3) ELISA Kit
Gene Names
Dpep3; MBD-3; MLZ-136; AI507039; 1700018F16Rik
Reactivity
Human
Synonyms
Dipeptidase 3 (DPEP3); Human Dipeptidase 3 (DPEP3) ELISA Kit; MBD3; DPEP3 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of DPEP3. No significant cross-reactivity or interference between DPEP3 and analogues was observed.
Sequence Length
493
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
< 0.156ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DPEP3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DPEP3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DPEP3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DPEP3 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to DPEP3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to DPEP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DPEP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DPEP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to DPEP3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to DPEP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DPEP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DPEP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,247 Da
NCBI Official Full Name
dipeptidase 3
NCBI Official Synonym Full Names
dipeptidase 3
NCBI Official Symbol
Dpep3
NCBI Official Synonym Symbols
MBD-3; MLZ-136; AI507039; 1700018F16Rik
NCBI Protein Information
dipeptidase 3
UniProt Protein Name
Dipeptidase 3
Protein Family
UniProt Gene Name
Dpep3
UniProt Synonym Gene Names
Mbd3; MBD-3; MLZ-136
Uniprot Description
Probable metalloprotease which hydrolyzes cystinyl-bis-glycine. May be involved in meiosis.
Research Articles on DPEP3
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Product Notes
The Human DPEP3 dpep3 (Catalog #AAA2705790) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2705790 ELISA Kit recognizes Human DPEP3. It is sometimes possible for the material contained within the vial of "Dipeptidase 3 (DPEP3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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