Rabbit anti-Human SGK3 Polyclonal Antibody | anti-SGK3 antibody

SGK3, NT (SGK3, CISK, SGKL, Serine/threonine-protein kinase Sgk3, Cytokine-independent survival kinase, Serum/glucocorticoid-regulated kinase 3, Serum/glucocorticoid-regulated kinase-like) (FITC)

Cat. Num. AAA6346980

• Gene Names: SGK3; CISK; SGK2; SGKL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SGK3; Polyclonal Antibody; NT (SGK3; CISK; SGKL; Serine/threonine-protein kinase Sgk3; Cytokine-independent survival kinase; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like) (FITC); Serum/glucocorticoid-regulated kinase-like; anti-SGK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SGK3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SGK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7~36 amino acids from the N-terminal region of human SGK3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-SKG3 Pab in A375 cell lysate. SGK3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(IHCdetection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50.)

Related Product Information for anti-SGK3 antibody

SGK3, a Ser/Thr protein kinase, is similar to serum-and glucocorticoid-induced protein kinase (SGK), but this gene product is not induced by serum or glucocorticoids. Expression is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK.

Product Categories/Family for anti-SGK3 antibody

Antibodies; Protein Kinases

References

Friedrich, B., et al., Pflugers Arch. 445(6):693-696 (2003). Embark, H.M., et al., Pflugers Arch. 445(5):601-606 (2003). Brickley, D.R., et al., J. Biol. Chem. 277(45):43064-43070 (2002). Gamper, N., et al., Pflugers Arch. 445(1):60-66 (2002). Dai, F., et al., Biochem. Biophys. Res. Commun. 293(4):1191-1196 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 75813626
• NCBI GeneID: 23678
• NCBI Accession #: NP_001028750.1
• NCBI GenBank Nucleotide #: NM_001033578.2
• UniProt Accession #: Q96BR1; Q9P1Q7; Q9UKG5; A8K5W3; B3KQC2
• Molecular Weight: 53,306 Da
• NCBI Official Full Name: serine/threonine-protein kinase Sgk3 isoform 1
• NCBI Official Synonym Full Names: serum/glucocorticoid regulated kinase family, member 3
• NCBI Official Symbol: SGK3
• NCBI Official Synonym Symbols: CISK; SGK2; SGKL
• NCBI Protein Information: serine/threonine-protein kinase Sgk3; cytokine-independent survival kinase
• UniProt Protein Name: Serine/threonine-protein kinase Sgk3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: SGK3
• UniProt Synonym Gene Names: CISK; SGKL
• UniProt Entry Name: SGK3_HUMAN

NCBI Description

This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

SGK3: Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes. Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner. Induced by estrogen/ER in breast cancer cells. Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER- positive breast tumors than ER-negative breast tumors. Two specific sites, one in the kinase domain (Thr-320) and the other in the C-terminal regulatory region (Ser- 486), need to be phosphorylated for its full activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; AGC group; SGK family

Chromosomal Location of Human Ortholog: 8q12

Cellular Component: recycling endosome; cytoplasmic membrane-bound vesicle; early endosome; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; potassium channel regulator activity; chloride channel regulator activity; sodium channel regulator activity; calcium channel regulator activity; ATP binding; phosphoinositide binding; protein kinase activity

Biological Process: regulation of transcription factor activity; response to stress; regulation of cell growth; transmembrane transport; protein amino acid phosphorylation; regulation of cell migration; regulation of cell proliferation