Mouse PARK7/Protein DJ-1 ELISA Kit | PARK7 elisa kit

Mouse PARK7/Protein DJ-1 ELISA Kit

Cat. Num. AAA2890864

• Gene Names: Park7; Dj1; DJ-1
• Reactivity: Mouse
• Synonyms: PARK7/Protein DJ-1; Mouse PARK7/Protein DJ-1 ELISA Kit; Protein DJ-1; Parkinson disease protein 7 homolog; Protein deglycase DJ-1; 3.1.2.-; PARK7 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse Protein/nucleic acid deglycase DJ-1
• Sequence Length: 189

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.312-20 ng/mL
• Sensitivity: < 0.125 ng/mL
• Intra-assay Precision: <=5.4%
• Inter-assay Precision: <=9.7%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PARK7 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55741460
• NCBI GeneID: 57320
• NCBI Accession #: NP_065594.2
• NCBI GenBank Nucleotide #: NM_020569.3
• UniProt Accession #: Q99LX0
• NCBI Official Full Name: protein/nucleic acid deglycase DJ-1
• NCBI Official Synonym Full Names: Parkinson disease (autosomal recessive, early onset) 7
• NCBI Official Symbol: Park7
• NCBI Official Synonym Symbols: Dj1; DJ-1
• NCBI Protein Information: protein/nucleic acid deglycase DJ-1
• UniProt Protein Name: Protein deglycase DJ-1
• Protein Family: Protein deglycase
• UniProt Gene Name: Park7
• UniProt Entry Name: PARK7_MOUSE

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: EC 3.4.-.-; Nuclear receptor co-regulator; Transcription regulation; Tumor suppressor

Cellular Component: axon; cell-cell adherens junction; chromatin; cytoplasm; cytosol; endoplasmic reticulum; mitochondrial intermembrane space; mitochondrial matrix; mitochondrial respiratory chain complex I; mitochondrion; neuron projection; nucleus; perinuclear region of cytoplasm; PML body

Molecular Function: androgen receptor binding; copper ion binding; cytokine binding; enzyme binding; identical protein binding; kinase binding; mercury ion binding; mRNA binding; oxidoreductase activity, acting on peroxide as acceptor; peptidase activity; peroxiredoxin activity; protein binding; protein homodimerization activity; receptor binding; RNA binding; superoxide dismutase copper chaperone activity; transcription coactivator activity; transcription factor binding

Biological Process: adult locomotory behavior; cell proliferation; detoxification of copper ion; detoxification of mercury ion; dopamine uptake; enzyme active site formation via L-cysteine sulfinic acid; glycolate biosynthetic process; hydrogen peroxide metabolic process; lactate biosynthetic process; membrane depolarization; membrane hyperpolarization; methylglyoxal metabolic process; mitochondrion organization and biogenesis; negative regulation of apoptosis; negative regulation of neuron apoptosis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein amino acid phosphorylation; negative regulation of protein binding; negative regulation of protein catabolic process; negative regulation of protein export from nucleus; negative regulation of protein kinase activity; negative regulation of protein sumoylation; negative regulation of protein ubiquitination; negative regulation of ubiquitin-protein ligase activity; positive regulation of interleukin-8 production; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; protein deglycosylation; protein stabilization; regulation of inflammatory response; regulation of mitochondrial membrane potential; regulation of neuron apoptosis; response to hydrogen peroxide; response to oxidative stress; synaptic transmission, dopaminergic