Typical Testing Data/Standard Curve (for reference only)
Mouse DNA topoisomerase 2-alpha ELISA Kit | Top2a elisa kit
Mouse DNA topoisomerase 2-alpha ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
TOP2A: a DNA topoisomerase. Controls of topological states of DNA by transient breakage and rejoining of DNA. Makes double-strand breaks. Four splice variant isoforms have been described.
Protein type: EC 5.99.1.3; Isomerase
Cellular Component: centriole; condensed chromosome; DNA topoisomerase complex (ATP-hydrolyzing); nuclear chromosome; nucleolus; nucleoplasm; nucleus; protein complex
Molecular Function: ATP binding; chromatin binding; DNA bending activity; DNA binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase activity; DNA-dependent ATPase activity; drug binding; enzyme binding; histone deacetylase binding; isomerase activity; magnesium ion binding; metal ion binding; nucleotide binding; protein C-terminus binding; protein heterodimerization activity; protein homodimerization activity; protein kinase C binding; sequence-specific DNA binding; structure-specific DNA binding; ubiquitin binding
Biological Process: apoptotic chromosome condensation; chromosome condensation; chromosome segregation; DNA ligation; DNA metabolic process; DNA topological change; embryonic cleavage; hemopoietic progenitor cell differentiation; positive regulation of apoptosis; positive regulation of retroviral genome replication; positive regulation of transcription from RNA polymerase II promoter; positive regulation of viral genome replication; regulation of circadian rhythm; resolution of meiotic joint molecules as recombinants; response to DNA damage stimulus; rhythmic process; sister chromatid segregation
Research Articles on Top2a
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Product Notes
The Mouse Top2a top2a (Catalog #AAA2887194) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2887194 ELISA Kit recognizes Mouse Top2a. It is sometimes possible for the material contained within the vial of "DNA topoisomerase 2-alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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