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DNA Topoisomerase-I 70kDa Recombinant Protein | TOP1 recombinant protein

Recombinant Human DNA Topoisomerase-I 70kDa

Gene Names
TOP1; TOPI
Applications
Western Blot
Purity
Greater than 95% as determined by SDS-PAGE.
Synonyms
DNA Topoisomerase-I 70kDa; Recombinant Human DNA Topoisomerase-I 70kDa; TOP1 70kDa Human; DNA Topoisomerase-I 70kDa Recombinant Human; DNA topoisomerase 1; EC 5.99.1.2; DNA topoisomerase I; TOP1; Scl-70; TOP1 70kDa; TOP1 recombinant protein
Ordering
For Research Use Only!
Host
Sf9 Insect Cells
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
Sterile Filtered clear solution
TOP1 70kDa is supplied in 20mM HEPES buffer pH-8.0, 500mM NaCl, and 20% glycerol.
Concentration
0.5-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. (varies by lot)
Sequence Length
765
Applicable Applications for TOP1 recombinant protein
Western Blot (WB)
Application Notes
Western blot with anti TOP1 70kDa autoantibody positive sample or monoclonal anti-hexa-His-tag antibody.
Immunological functions

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checkerboard analysis of positive/negative samples) and immunodot test with positive/negative samples.
Coating concentration
0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional group
Preparation and Storage
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Related Product Information for TOP1 recombinant protein
Description: Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 72KDa (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag.
Product Categories/Family for TOP1 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DNA topoisomerase 1
NCBI Official Synonym Full Names
topoisomerase (DNA) I
NCBI Official Symbol
TOP1
NCBI Official Synonym Symbols
TOPI
NCBI Protein Information
DNA topoisomerase 1; type I DNA topoisomerase
UniProt Protein Name
DNA topoisomerase 1
Protein Family
UniProt Gene Name
TOP1
UniProt Entry Name
TOP1_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

Uniprot Description

TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.

Protein type: Oncoprotein; Nucleolus; EC 5.99.1.2; Isomerase

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: nucleoplasm; nuclear chromosome; nucleolus; perikaryon; nucleus

Molecular Function: DNA topoisomerase (ATP-hydrolyzing) activity; protein binding; DNA binding; chromatin binding; DNA topoisomerase type I activity

Biological Process: chromatin remodeling; response to drug; circadian rhythm; DNA topological change; viral reproduction; programmed cell death; circadian regulation of gene expression; DNA replication; phosphorylation; embryonic cleavage; chromosome segregation

Research Articles on TOP1

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Product Notes

The TOP1 top1 (Catalog #AAA145206) is a Recombinant Protein produced from Sf9 Insect Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DNA Topoisomerase-I 70kDa can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blot with anti TOP1 70kDa autoantibody positive sample or monoclonal anti-hexa-His-tag antibody. Researchers should empirically determine the suitability of the TOP1 top1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Topoisomerase-I 70kDa, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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