DNA Topoisomerase-I 70kDa Recombinant Protein | TOP1 recombinant protein

Recombinant Human DNA Topoisomerase-I 70kDa

Cat. Num. AAA145206

• Gene Names: TOP1; TOPI
• Applications: Western Blot
• Purity: Greater than 95% as determined by SDS-PAGE.
• Synonyms: DNA Topoisomerase-I 70kDa; Recombinant Human DNA Topoisomerase-I 70kDa; TOP1 70kDa Human; DNA Topoisomerase-I 70kDa Recombinant Human; DNA topoisomerase 1; EC 5.99.1.2; DNA topoisomerase I; TOP1; Scl-70; TOP1 70kDa; TOP1 recombinant protein

• Host: Sf9 Insect Cells
• Purity/Purification: Greater than 95% as determined by SDS-PAGE.
• Form/Format: Sterile Filtered clear solution; TOP1 70kDa is supplied in 20mM HEPES buffer pH-8.0, 500mM NaCl, and 20% glycerol.
• Concentration: 0.5-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. (varies by lot)
• Sequence Length: 765

• Applicable Applications for TOP1 recombinant protein: Western Blot (WB)
• Application Notes: Western blot with anti TOP1 70kDa autoantibody positive sample or monoclonal anti-hexa-His-tag antibody.
• Immunological functions: 1. Binds IgG-type human auto-antibodies.; 2. Standard ELISA test (checkerboard analysis of positive/negative samples) and immunodot test with positive/negative samples.
• Coating concentration: 0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional group
• Preparation and Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Related Product Information for TOP1 recombinant protein

Description: Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 72KDa (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag.

Product Categories/Family for TOP1 recombinant protein

Enzymes; Isomerase

NCBI and Uniprot Product Information

• NCBI GI #: 11225260
• NCBI GeneID: 7150
• NCBI Accession #: NP_003277.1
• NCBI GenBank Nucleotide #: NM_003286.2
• UniProt Accession #: P11387; O43256; Q12855; Q12856; Q15610; Q5TFY3; Q9UJN0; A8KA78; E1P5W3
• NCBI Official Full Name: DNA topoisomerase 1
• NCBI Official Synonym Full Names: topoisomerase (DNA) I
• NCBI Official Symbol: TOP1
• NCBI Official Synonym Symbols: TOPI
• NCBI Protein Information: DNA topoisomerase 1; type I DNA topoisomerase
• UniProt Protein Name: DNA topoisomerase 1
• Protein Family: DNA topoisomerase
• UniProt Gene Name: TOP1
• UniProt Entry Name: TOP1_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

Uniprot Description

TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.

Protein type: Oncoprotein; Nucleolus; EC 5.99.1.2; Isomerase

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: nucleoplasm; nuclear chromosome; nucleolus; perikaryon; nucleus

Molecular Function: DNA topoisomerase (ATP-hydrolyzing) activity; protein binding; DNA binding; chromatin binding; DNA topoisomerase type I activity

Biological Process: chromatin remodeling; response to drug; circadian rhythm; DNA topological change; viral reproduction; programmed cell death; circadian regulation of gene expression; DNA replication; phosphorylation; embryonic cleavage; chromosome segregation