Mouse DNA topoisomerase 1 ELISA Kit | Top1 elisa kit

Mouse DNA topoisomerase 1 ELISA Kit

Cat. Num. AAA764536

• Gene Names: TOP1; TOPI
• Reactivity: Mouse
• Synonyms: DNA topoisomerase 1; Mouse DNA topoisomerase 1 ELISA Kit; Top1/DNA topoisomerase I; Top1 elisa kit

• Reactivity: Mouse
• Sequence Length: 765

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Top1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Top1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Top1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Top1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Top1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 11225260
• NCBI GeneID: 7150
• NCBI Accession #: NP_003277.1
• NCBI GenBank Nucleotide #: NM_003286.2
• Molecular Weight: 90,726 Da
• NCBI Official Full Name: DNA topoisomerase 1
• NCBI Official Synonym Full Names: topoisomerase (DNA) I
• NCBI Official Symbol: TOP1
• NCBI Official Synonym Symbols: TOPI
• NCBI Protein Information: DNA topoisomerase 1
• UniProt Protein Name: DNA topoisomerase 1
• Protein Family: DNA topoisomerase
• UniProt Gene Name: TOP1
• UniProt Entry Name: TOP1_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

Uniprot Description

TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.

Protein type: EC 5.99.1.2; Isomerase; Nucleolus; Oncoprotein

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: nuclear chromosome; nucleolus; nucleoplasm; nucleus; perikaryon

Molecular Function: chromatin binding; DNA binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase type I activity; protein binding

Biological Process: cellular protein metabolic process; chromatin remodeling; chromosome segregation; circadian regulation of gene expression; circadian rhythm; DNA replication; DNA topological change; embryonic cleavage; phosphorylation; post-translational protein modification; programmed cell death; protein sumoylation; response to drug; viral reproduction