Immunohistochemistry (IHC)
(Immunohistochemical staining of normal human tonsil tissue using TOP1 antibody at 10 ug/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain.)
Rabbit anti-Human DNA Topoisomerase 1 Polyclonal Antibody | anti-TOP1 antibody
DNA Topoisomerase 1
Immunohistochemistry (IHC)
(Immunohistochemical staining of normal human tonsil tissue using TOP1 antibody at 10 ug/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of normal human tonsil tissue using TOP1 antibody at 10 ug/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
By similarity. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Ref.1 Ref.17 Ref.29 Ref.32
Catalytic activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. Ref.1 Ref.29 Ref.32
Enzyme regulation: Specifically inhibited by camptothecin (CPT), a plant alkaloid with antitumor activity.
Subunit structure: Monomer. Interacts with SV40 Large T antigen; this interactions allows viral DNA replication. Ref.15
Subcellular location: Nucleus › nucleolus. Nucleus › nucleoplasm. Note: Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli. Ref.13
Tissue specificity: Endothelial cells. Ref.17
Post-translational modification: Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. Ref.13Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.
Involvement in disease: A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
Miscellaneous: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.
Sequence similarities: Belongs to the type IB topoisomerase family.
Sequence caution: The sequence CAA36834.1 differs from that shown. Reason: Erroneous gene model prediction.
Research Articles on TOP1
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Product Notes
The TOP1 top1 (Catalog #AAA395914) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNA Topoisomerase 1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA Topoisomerase 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the TOP1 top1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Topoisomerase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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