Rat topoisomerase (DNA) I ELISA Kit | TOP1 elisa kit
Rat DNA topoisomerase 1, TOP1 ELISA Kit
NCBI and Uniprot Product Information
NCBI Description
human homolog catalyzes the transient breakage and rejoining of DNA; plays a role in nucleotide excision repair [RGD, Feb 2006]
Uniprot Description
TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.
Protein type: EC 5.99.1.2; Oncoprotein; Nucleolus; Isomerase
Cellular Component: nucleoplasm; nuclear chromosome; dense fibrillar component; cytoplasm; nucleolus; fibrillar center; perikaryon; nucleus
Molecular Function: DNA topoisomerase (ATP-hydrolyzing) activity; DNA binding; chromatin DNA binding; protein complex binding; chromatin binding; DNA topoisomerase type I activity
Biological Process: circadian rhythm; response to temperature stimulus; response to drug; chromatin remodeling; organ regeneration; DNA topological change; response to cAMP; response to gamma radiation; circadian regulation of gene expression; DNA replication; embryonic cleavage; chromosome segregation; rRNA transcription
Research Articles on TOP1
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Product Notes
The Rat TOP1 top1 (Catalog #AAA9339044) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9339044 ELISA Kit recognizes Rat TOP1. It is sometimes possible for the material contained within the vial of "topoisomerase (DNA) I, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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