Cardiac Troponin T (TnT) Recombinant Protein | TNNT2 recombinant protein

Recombinant Human Cardiac Troponin T (TnT)

Cat. Num. AAA197054

• Gene Names: TNNT2; CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6; MGC3889
• Purity: Greater than 90% as determined by SDS-PAGE
• Synonyms: Cardiac Troponin T (TnT); Recombinant Human Cardiac Troponin T (TnT); TNNT2 recombinant protein

• Host: E Coli
• Specificity: Human Cardiac Troponin T (TnT)
• Purity/Purification: Greater than 90% as determined by SDS-PAGE
• Form/Format: Sterile-filtered solution in 20mM Tris, pH 8.0, 10% glycerol. Purified by proprietary chromatographic techniques.
• Sequence: MGSSHHHHHH SSGLVPRGSH MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK

• Accession Number: P45379.3
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store at -20 degree C for longer periods of time. For long term storage, additon of 0.1% human serum albumin or bovine serum albumin is recommended. Avoid multiple freeze-thaw cycles.

Related Product Information for TNNT2 recombinant protein

Troponin T (TnT) is the tropomyosinbinding subunit of the troponin complex which is situated on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentrations. Mutations in the TNNT2 gene are associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy and hypertrophic cardiomyopathy.

Recombinant Human TnT produced in E. coli is a single, non-glycosylated polypeptide chain containing 305 amino acids (aa 1-285) with a molecular weight of 36.4 kDa. It is fused to a 20-amino acid His-tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 48255877
• NCBI GeneID: 7139
• NCBI Accession #: NP_000355.2
• NCBI GenBank Nucleotide #: NM_000364.2
• UniProt Accession #: P45379; O60214; Q99596; Q99597; Q9UM96; A2TDB9; A8K3K6; Q15607; Q15608; Q7Z554; Q8IZA1; Q9BUF6
• Molecular Weight: 35,924 Da
• NCBI Official Full Name: troponin T, cardiac muscle isoform 1
• NCBI Official Synonym Full Names: troponin T type 2 (cardiac)
• NCBI Official Symbol: TNNT2
• NCBI Official Synonym Symbols: CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6; MGC3889
• NCBI Protein Information: troponin T, cardiac muscle; OTTHUMP00000033864; OTTHUMP00000033865; OTTHUMP00000033866; OTTHUMP00000033867; OTTHUMP00000033870; OTTHUMP00000218095; troponin T2, cardiac; cardiac muscle troponin T; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant)
• UniProt Protein Name: Troponin T, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT2
• UniProt Entry Name: TNNT2_HUMAN

NCBI Description

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq]

Uniprot Description

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament

Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; actin binding; tropomyosin binding

Biological Process: atrial cardiac muscle morphogenesis; positive regulation of ATPase activity; metabolic process; regulation of muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2