Troponin T Recombinant Protein | TNNT2 recombinant protein

Troponin T, 1-285aa, Human, His tag, E Coli

Cat. Num. AAA203330

• Gene Names: TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Troponin T; 1-285aa; Human; His tag; E Coli; Troponin T type 2 cardiac isoform 3; Troponin T type 2; cardiac isoform 3; CMH2; CMPD2; cTnT; RCM3; TnTC; cardiac isoform 3 Cardiac muscle troponin T; MGC3889; TNNT 2; TNNT2; Cardiomyopathy hypertrophic 2; Cardiomyopathy dilated 1D (autosomal dominant); CMD1D; Troponin T cardiac muscle; Troponin T type 2 (cardiac); Troponin T type 2 cardiac; Troponin T2 cardiac.; TNNT2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 50% glycerol, 0.5M NaCl, 0.1mM PMSF, 1mM DTT, 100mM imidazole
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK
• Sequence Length: 285

• Applicable Applications for TNNT2 recombinant protein: SDS-PAGE
• Preparation and Storage: Can be stored at 4°C short term (1-2 weeks).; For long term storage, aliquot and store at -20°C or -70°C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TNNT2 recombinant protein

Troponin T, also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with restrictive and dilated cardiomyopathy. Recombinant human TNNT2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for TNNT2 recombinant protein

Cytoskeleton

References

Yu H., et al. (2009), Proteomics. 9 (3):504-11.; Morita H., et al. (2008). N Engl J Med. 358 (18):1899-908.;

NCBI and Uniprot Product Information

• NCBI GI #: 48255881
• NCBI GeneID: 7139
• NCBI Accession #: NP_001001431
• NCBI GenBank Nucleotide #: NM_001001431.2
• UniProt Accession #: P45379; O60214; Q99596; Q99597; Q9BUF6; Q9UM96; A2TDB9; A8K3K6
• Molecular Weight: 36.4 kDa (305aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: troponin T, cardiac muscle isoform 3
• NCBI Official Synonym Full Names: troponin T2, cardiac type
• NCBI Official Symbol: TNNT2
• NCBI Official Synonym Symbols: CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• NCBI Protein Information: troponin T, cardiac muscle
• UniProt Protein Name: Troponin T, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT2
• UniProt Synonym Gene Names: TnTc; cTnT

NCBI Description

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.