Rat Troponin I, fast skeletal muscle ELISA Kit | Tnni2 elisa kit

Rat Troponin I, fast skeletal muscle ELISA Kit

Cat. Num. AAA762589

• Gene Names: TNNI2; TnI
• Reactivity: Rat
• Synonyms: Troponin I; fast skeletal muscle; Rat Troponin I; fast skeletal muscle ELISA Kit; Tnni2/Troponin I; fast-twitch isoform; Tnni2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tnni2 . No significant cross-reactivity or interference between Tnni2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross - reactivity detection between Tnni2 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 182

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 31.2-2000pg/ml
• Sensitivity: <18.75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tnni2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tnni2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Tnni2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Tnni2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Tnni2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Tnni2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Tnni2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 130493079
• NCBI GeneID: 100009581
• NCBI Accession #: NP_001076252.1
• NCBI GenBank Nucleotide #: NM_001082783.1
• Molecular Weight: 21,214 Da
• NCBI Official Full Name: troponin I, fast skeletal muscle
• NCBI Official Symbol: TNNI2
• NCBI Official Synonym Symbols: TnI
• NCBI Protein Information: troponin I, fast skeletal muscle
• UniProt Protein Name: Troponin I, fast skeletal muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI2
• UniProt Entry Name: TNNI2_RABIT

Uniprot Description

TNNI2: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis