Rabbit anti-Human Kallikrein 5 Polyclonal Antibody | anti-KLK5 antibody

Kallikrein 5 (Kallikrein-5, KLK5, Kallikrein-like Protein 2, KLKL2, KLK-L2, Kallikrein-related Peptidase 5, Stratum Corneum Tryptic Enzyme, SCTE, UNQ570/PRO1132) (MaxLight 405)

Cat. Num. AAA6484046

• Gene Names: KLK5; SCTE; KLKL2; KLK-L2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Kallikrein 5; Polyclonal Antibody; Kallikrein 5 (Kallikrein-5; KLK5; Kallikrein-like Protein 2; KLKL2; KLK-L2; Kallikrein-related Peptidase 5; Stratum Corneum Tryptic Enzyme; SCTE; UNQ570/PRO1132) (MaxLight 405); EC=3.4.21.-; anti-KLK5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KLK5.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
• Sequence Length: 293

• Applicable Applications for anti-KLK5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide composed of corresponding to a portion of amino acids within aa152-181from the central region of human KLK5 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-KLK5 Pab in mouse brain tissue lysate.KLK5(arrow) was detected using the purified Pab.)

Western Blot (WB)

(KLK5 Antibody (L159) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the KLK5 antibody detected the KLK5 protein (arrow).)

Product Categories/Family for anti-KLK5 antibody

Antibodies; Kallikrein

References

1. Planque, C., et al., Biochem. Biophys. Res. Commun. 329(4):1260-1266 (2005). 2. Michael, I.P., et al., J. Biol. Chem. 280(15):14628-14635 (2005). 3. Ishida-Yamamoto, A., et al., J. Invest. Dermatol. 124(2):360-366 (2005). 4. Brattsand, M., et al., J. Invest. Dermatol. 124(1):198-203 (2005). 5. Caubet, C., et al., J. Invest. Dermatol. 122(5):1235-1244 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 6912644
• NCBI GeneID: 25818
• NCBI Accession #: NP_036559
• NCBI GenBank Nucleotide #: NP_036559.1
• UniProt Accession #: Q9Y337
• Molecular Weight: 32.02
• NCBI Official Full Name: kallikrein-5 preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 5
• NCBI Official Symbol: KLK5
• NCBI Official Synonym Symbols: SCTE; KLKL2; KLK-L2
• NCBI Protein Information: kallikrein-5
• UniProt Protein Name: Kallikrein-5
• Protein Family: Kallikrein
• UniProt Gene Name: KLK5
• UniProt Synonym Gene Names: SCTE; KLK-L2
• UniProt Entry Name: KLK5_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. The encoded protein is secreted and may be involved in desquamation in the epidermis. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK5: May be involved in desquamation. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: EC 3.4.21.-; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular space

Molecular Function: peptidase activity; protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: epidermis development; positive regulation of G-protein coupled receptor protein signaling pathway; proteolysis