Human Troponin I, slow skeletal muscle ELISA Kit | TNNI1 elisa kit

Human Troponin I, slow skeletal muscle ELISA Kit

Cat. Num. AAA765801

• Gene Names: TNNI1; TNN1; SSTNI
• Reactivity: Human
• Synonyms: Troponin I; slow skeletal muscle; Human Troponin I; slow skeletal muscle ELISA Kit; TNNI1 (Troponin I Type 1; Slow Skeletal); TNNI1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNNI1 . No significant cross-reactivity or interference between TNNI1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between TNNI1 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 187

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 46.875-3000pg/ml
• Sensitivity: <28.125pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNNI1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNNI1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNNI1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TNNI1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TNNI1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TNNI1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TNNI1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 56682969
• NCBI GeneID: 7135
• NCBI Accession #: NP_003272.3
• NCBI GenBank Nucleotide #: NM_003281.3
• Molecular Weight: 21,692 Da
• NCBI Official Full Name: troponin I, slow skeletal muscle
• NCBI Official Synonym Full Names: troponin I1, slow skeletal type
• NCBI Official Symbol: TNNI1
• NCBI Official Synonym Symbols: TNN1; SSTNI
• NCBI Protein Information: troponin I, slow skeletal muscle
• UniProt Protein Name: Troponin I, slow skeletal muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI1
• UniProt Entry Name: TNNI1_HUMAN

NCBI Description

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: cytosol; troponin complex

Molecular Function: actin binding; metal ion binding; protein binding

Biological Process: cardiac muscle contraction; muscle filament sliding; regulation of striated muscle contraction; skeletal muscle contraction; transition between fast and slow fiber; ventricular cardiac muscle morphogenesis