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Typical Testing Data

Human Troponin I, fast skeletal muscle ELISA Kit | TNNI2 elisa kit

Human Troponin I, fast skeletal muscle ELISA Kit

Gene Names
TNNI2; TnI
Reactivity
Human
Synonyms
Troponin I; fast skeletal muscle; Human Troponin I; fast skeletal muscle ELISA Kit; TNNI2/Troponin I; fast-twitch isoform; TNNI2 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
182
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for TNNI2 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human TNNI2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TNNI2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNNI2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human TNNI2can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,214 Da
NCBI Official Full Name
troponin I, fast skeletal muscle
NCBI Official Symbol
TNNI2
NCBI Official Synonym Symbols
TnI
NCBI Protein Information
troponin I, fast skeletal muscle
UniProt Protein Name
Troponin I, fast skeletal muscle
Protein Family
UniProt Gene Name
TNNI2
UniProt Entry Name
TNNI2_RABIT

Uniprot Description

TNNI2: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis

Research Articles on TNNI2

Similar Products

Product Notes

The Human TNNI2 tnni2 (Catalog #AAA765786) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA765786 ELISA Kit recognizes Human TNNI2. It is sometimes possible for the material contained within the vial of "Troponin I, fast skeletal muscle, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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