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Western Blot (WB)
(Western Blot analysis with human PlGF1 and PlGF2. Purified recombinant human PlGF1 (red: 500ng, non-red: 1000ng) and PlGF2 (red: 250ng, non-red: 500ng) were loaded in 15% SDS-PAGE under reducing and non-reducing conditions. As primary antibody mouse anti-human PlGF2 #D1 was used in a concentration of 0.5µg/ml. The detection was carried out with a secondary, AP-conjugated anti-mouse Fc antibody (Dianova, 1:1000). NOTE: The anti-human PlGF-2 #D1 antibody recognizes solely the PlGF2 isoform.)
anti-Human PlGF-2 Monoclonal Antibody | anti-PlGF-2 antibody
Mouse Anti-Human PlGF-2
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Synonyms
PlGF-2; Monoclonal Antibody; Mouse Anti-Human PlGF-2; PlGF; placental growth factor; anti-PlGF-2 antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
A6D1
Form/Format
lyophilized
Applicable Applications for anti-PlGF-2 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Buffer
PBS
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Antigen
Recombinant human PlGF-2 (#MBS691507)
Preparation and Storage
The lyophilized antibody is stable for at least 2 years at -20 degree C. After sterile reconstitution the antibody is stable at 2-8 degree C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20 degree C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
Western Blot (WB)
(Western Blot analysis with human PlGF1 and PlGF2. Purified recombinant human PlGF1 (red: 500ng, non-red: 1000ng) and PlGF2 (red: 250ng, non-red: 500ng) were loaded in 15% SDS-PAGE under reducing and non-reducing conditions. As primary antibody mouse anti-human PlGF2 #D1 was used in a concentration of 0.5µg/ml. The detection was carried out with a secondary, AP-conjugated anti-mouse Fc antibody (Dianova, 1:1000). NOTE: The anti-human PlGF-2 #D1 antibody recognizes solely the PlGF2 isoform.)
Western Blot (WB)
(Western Blot analysis with human PlGF1 and PlGF2. Purified recombinant human PlGF1 (red: 500ng, non-red: 1000ng) and PlGF2 (red: 250ng, non-red: 500ng) were loaded in 15% SDS-PAGE under reducing and non-reducing conditions. As primary antibody mouse anti-human PlGF2 #D1 was used in a concentration of 0.5µg/ml. The detection was carried out with a secondary, AP-conjugated anti-mouse Fc antibody (Dianova, 1:1000). NOTE: The anti-human PlGF-2 #D1 antibody recognizes solely the PlGF2 isoform.)
Western Blot (WB)
(Western Blot analysis with PlGF. Purified recombinant human PlGF2 (500 -10 ng/lane), PlGF1 (1000 ng/lane), and mouse and rat PlGF (500ng/lane) all derived from insect cells were loaded in 12.5% SDS-PAGE under reducing conditions. As primary antibody mouse anti-human PlGF2 #D1 was used in a concentration of 2µg/ml. The detection was carried out with a secondary, AP-conjugated anti-mouse Fc antibody. NOTE: There is no cross reaction with human PlGF1, mouse and rat PlGF.)
Western Blot (WB)
(Western Blot analysis with PlGF. Purified recombinant human PlGF2 (500 -10 ng/lane), PlGF1 (1000 ng/lane), and mouse and rat PlGF (500ng/lane) all derived from insect cells were loaded in 12.5% SDS-PAGE under reducing conditions. As primary antibody mouse anti-human PlGF2 #D1 was used in a concentration of 2µg/ml. The detection was carried out with a secondary, AP-conjugated anti-mouse Fc antibody. NOTE: There is no cross reaction with human PlGF1, mouse and rat PlGF.)
Immunofluorescence (IF)
(IF staining of human PlGF1 and PlGF2 in Sf9 cells expressing either PlGF1 or PlGF2. Upper lane: mouse anti-human PlGF2 #D1 recognizing only PlGF2 and lower lane: mouse anti-human PlGF #178/G10 recognizing both isoforms of PlGF. )
Immunofluorescence (IF)
(IF staining of human PlGF1 and PlGF2 in Sf9 cells expressing either PlGF1 or PlGF2. Upper lane: mouse anti-human PlGF2 #D1 recognizing only PlGF2 and lower lane: mouse anti-human PlGF #178/G10 recognizing both isoforms of PlGF. )
ELISA (EIA)
(ELISA with recombinant human PlGF1 and PlGF2 derived from insect cells. Both proteins were coated to a 96-well plate in increasing amounts. The mouse anti-human PlGF #178/G10 antibody [recognizing both isoforms and the mouse anti-human PlGF2 #D1 antibody recognizing specifically PlGF2 were added with a concentration of 0.5µg/ml. Detection was carried out with an anti-mouse Biotin antibody (Dianova).)
ELISA (EIA)
(ELISA with recombinant human PlGF1 and PlGF2 derived from insect cells. Both proteins were coated to a 96-well plate in increasing amounts. The mouse anti-human PlGF #178/G10 antibody [recognizing both isoforms and the mouse anti-human PlGF2 #D1 antibody recognizing specifically PlGF2 were added with a concentration of 0.5µg/ml. Detection was carried out with an anti-mouse Biotin antibody (Dianova).)
Related Product Information for anti-PlGF-2 antibody
Placenta Growth Factor-2 (PlGF-2), a 22 kDa protein consisting of 152 amino acid residues is produced as a homodimer. PlGF is a polypeptide growth factor and a member of the platelet-derived growth factor family but more related to vascular endothelial growth factor (VEGF). PlGF acts only as a weak mitogen for those cell types possessing receptors for binding (e.g. vascular endothelial cells). At least one high-affinity receptor for PlGF (FLT-1 or VEGF-R1) has been demonstrated in different primary cell types (e.g. human umbilical vein endothelial cells and monocytes). In addition to its action as a weak mitogen it is also a chemoattractant for monocytes and endothelial cells. Three different proteins are generated by differential splicing of the human PlGF gene: PlGF-1 (131aa native chain), PlGF-2 (152aa) and PlGF-3 (203aa). All 3 mitogens are secretable proteins, but PlGF-2 can bind to heparin with high affinity. PlGF is apparently a homodimer, but preparations of PlGF show some heterogeneity on SDS gels depending of the varying degrees of glycosylation. All dimeric forms possess similar biological activities. Heterodimers between VEGF and PlGF are mitogenic for endothelial cells and have strong angiogenic activity in vivo (e.g. in the CAM assay or in the cornea pocket assay). Different cells and tissues (e.g. placenta) express PlGF-1 and PlGF-2 at different rates. A much related protein of PlGF is VEGF with about 53% homology and VEGF-B with similar biological activities.
NCBI and Uniprot Product Information
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Product Notes
The PlGF-2 (Catalog #AAA692571) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human PlGF-2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PlGF-2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the PlGF-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PlGF-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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