Fas/TNFRSF6/CD95 Active Protein | Fas active protein

Recombinant Mouse Fas/TNFRSF6/CD95 Protein (His Tag)

Cat. Num. AAA2556800

• Gene Names: Fas; lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Fas/TNFRSF6/CD95; Recombinant Mouse Fas/TNFRSF6/CD95 Protein (His Tag); Tumor Necrosis Factor Receptor Superfamily Member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD95; Fas; TNFRSF6; Fas active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 327

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fragment: Met 1-Arg 169
• Fusion Tag: C-6His
• Activity: Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells.The ED50 for this effect is typically 1-8 mug/mL in the presence of 20 ng/mL recombinant human Fas ligand.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

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Related Product Information for Fas active protein

Protein Construction: Recombinant Mouse Apoptosis-mediating Surface Antigen FAS is produced by our Mammalian expression system and the target gene encoding Gln22-Arg169 is expressed with a 6His tag at the C-terminus.

Background: Mouse Apoptosis-mediating surface antigen FAS (Fas) belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6. Mouse Fas contains 1 death domain and 3 TNFR-Cys repeats. It detected in various tissues including thymus, liver, lung, heart, and adult ovary. As a receptor for TNFSF6/FASLG, The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

NCBI and Uniprot Product Information

• NCBI GI #: 226443049
• NCBI GeneID: 14102
• NCBI Accession #: NP_032013.2
• NCBI GenBank Nucleotide #: NM_007987.2
• UniProt Accession #: P25446; Q6GT31; Q9DCQ1
• Molecular Weight: Molecular Mass: 17.4kDa; Actual Protein Molecular Mass: 25-35kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas (TNF receptor superfamily member 6)
• NCBI Official Symbol: Fas
• NCBI Official Synonym Symbols: lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Fas
• UniProt Synonym Gene Names: Apt1; Tnfrsf6

Uniprot Description

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both ().