Mouse Cyclin-dependent kinase 12 ELISA Kit | Cdk12 elisa kit

Mouse Cyclin-dependent kinase 12 ELISA Kit

Cat. Num. AAA288487

• Gene Names: Cdk12; Crk7; Pksc; Crkrs; AI646528; D11Ertd752e; 1810022J16Rik
• Reactivity: Mouse
• Synonyms: Cyclin-dependent kinase 12; Mouse Cyclin-dependent kinase 12 ELISA Kit; Cdk12 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Cyclin-dependent kinase 12. No significant cross-reactivity or interference was observed.
• Sequence Length: 1239

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/ml
• Sensitivity: 41 pg/mL
• Intra-Assay CV: <=4.6%
• Inter-Assay CV: <=7.6%
• Recovery: 97%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cdk12 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin-dependent kinase 12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cyclin-dependent kinase 12 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyclin-dependent kinase 12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cyclin-dependent kinase 12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157816935
• NCBI GeneID: 69131
• NCBI Accession #: NP_001103096.1
• NCBI GenBank Nucleotide #: NM_001109626.1
• UniProt Accession #: Q14AX6; Q6ZQ27; Q8R457; A2A530; A2A531; B1AQH7
• Molecular Weight: 162,755 Da
• NCBI Official Full Name: cyclin-dependent kinase 12 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 12
• NCBI Official Symbol: Cdk12
• NCBI Official Synonym Symbols: Crk7; Pksc; Crkrs; AI646528; D11Ertd752e; 1810022J16Rik
• NCBI Protein Information: cyclin-dependent kinase 12
• UniProt Protein Name: Cyclin-dependent kinase 12
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk12
• UniProt Synonym Gene Names: Crk7; Crkrs; Kiaa0904; CrkRS; CDC2-related protein kinase 7
• UniProt Entry Name: CDK12_MOUSE

Uniprot Description

CDK12: a Ser/Thr protein kinase of the CMGC group and CDK family. Phosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Interacts with CCNL1 and CCNL2. Colocalized with nuclear speckles throughout interphase. Has a highly phosphorylated arginine/serine-rich (RS) domain N-terminal to the kinase domain. Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Kinase, protein; Protein kinase, CMGC; RNA splicing; EC 2.7.11.22; Motility/polarity/chemotaxis; EC 2.7.11.23; CMGC group; CDK family; CDK/CRK7 subfamily; CRK7 subfamily

Cellular Component: nuclear cyclin-dependent protein kinase holoenzyme complex; nuclear speck; nucleolus; nucleus

Molecular Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; kinase activity; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity; transferase activity

Biological Process: mRNA processing; phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of MAP kinase activity; regulation of RNA splicing; RNA splicing