Human protease, serine, 7 (enterokinase) ELISA Kit | PRSS7 elisa kit

Human Enteropeptidase, PRSS7 ELISA Kit

Cat. Num. AAA920466

• Gene Names: TMPRSS15; ENTK; PRSS7
• Reactivity: Human
• Synonyms: protease; serine; 7 (enterokinase); Human Enteropeptidase; PRSS7 ELISA Kit; Human Enteropeptidase (PRSS7) ELISA kit; ENTK; MGC133046; enterokinase; enteropeptidase; PRSS7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human PRSS7. No significant cross-reactivity or interference between human PRSS7 and analogues was observed.
• Sequence Length: 1019

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: < 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRSS7 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRSS7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRSS7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 223942069
• NCBI GeneID: 5651
• NCBI Accession #: NP_002763.2
• NCBI GenBank Nucleotide #: NM_002772.2
• UniProt Accession #: P98073; Q2NKL7
• Molecular Weight: 112,935 Da
• NCBI Official Full Name: enteropeptidase
• NCBI Official Synonym Full Names: transmembrane protease, serine 15
• NCBI Official Symbol: TMPRSS15
• NCBI Official Synonym Symbols: ENTK; PRSS7
• NCBI Protein Information: enteropeptidase; serine protease 7; enterokinase catalytic subunit; protease, serine, 7 (enterokinase)
• UniProt Protein Name: Enteropeptidase
• UniProt Gene Name: TMPRSS15
• UniProt Synonym Gene Names: ENTK; PRSS7
• UniProt Entry Name: ENTK_HUMAN

NCBI Description

This gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive. [provided by RefSeq, Jul 2008]

Uniprot Description

PRSS7: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD); a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. Belongs to the peptidase S1 family.

Protein type: Protease; EC 3.4.21.9; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: integral to membrane; brush border

Molecular Function: serine-type endopeptidase activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; proteolysis

Disease: Enterokinase Deficiency