Human TIMP-2 ELISA Kit | TIMP2 elisa kit

Human TIMP-2 PicoKine ELISA Kit

Cat. Num. AAA175859

• Gene Names: TIMP2; DDC8; CSC-21K
• Reactivity: Human
• Synonyms: TIMP-2; Human TIMP-2 PicoKine ELISA Kit; Metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; CSC 21K; CSC21K; D11Bwg1104e; DDC8; Metalloproteinase inhibitor 2 precursor; MGC105282; OTTMUSP00000004170; OTTMUSP00000004171; RP23-394O9.2; TIMP 2; Tissue inhibitor of metalloproteinase 2; Tissue inhibitor of metalloproteinases 2; TIMP2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human TIMP-2
• Sequence Length: 220

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and saliva
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 2pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human TIMP-2 PicoKine ELISA Kit standard curve )

Related Product Information for TIMP2 elisa kit

Principle of the assay: human TIMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-2 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-2 amount of sample captured in plate.
Background: TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

NCBI and Uniprot Product Information

• NCBI GI #: 135854
• NCBI GeneID: 7077
• UniProt Accession #: P16035; Q16121; Q93006; Q9UDF7; Q96MC4
• Molecular Weight: 24,399 Da
• NCBI Official Full Name: Metalloproteinase inhibitor 2
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 2
• NCBI Official Symbol: TIMP2
• NCBI Official Synonym Symbols: DDC8; CSC-21K
• NCBI Protein Information: metalloproteinase inhibitor 2; TIMP-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2
• UniProt Protein Name: Metalloproteinase inhibitor 2
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP2
• UniProt Synonym Gene Names: TIMP-2
• UniProt Entry Name: TIMP2_HUMAN

NCBI Description

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis; Cell development/differentiation; Inhibitor

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extracellular space; proteinaceous extracellular matrix; growth cone; cell surface; cell soma; extracellular region; basement membrane

Molecular Function: integrin binding; metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding; enzyme activator activity

Biological Process: response to drug; regulation of Rap protein signal transduction; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; central nervous system development; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging