Human TIMP-2 ELISA Kit | TIMP-2 elisa kit

Human TIMP-2 PicoKine Fast ELISA Kit

Cat. Num. AAA1752733

• Gene Names: TIMP2; DDC8; CSC-21K
• Reactivity: Human
• Synonyms: TIMP-2; Human TIMP-2 PicoKine Fast ELISA Kit; Human TIMP-2; Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP2; TIMP-2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human TIMP2
• Sequence Length: 220

• Samples: Cell culture supernates, serum, plasma(heparin, EDTA) and saliva
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIMP-2 elisa kit

Principle of the Assay: The Picokine™ Human TIMP2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TIMP2 with a 96-well strip plate that is pre-coated with antibody specific for TIMP2. The detection antibody is a biotinylated antibody specific for TIMP2. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TIMP2 with immunogen: Expression system for standard: CHO; Immunogen sequence: C27-P220. The kit is analytically validated with ready to use reagents. To measure Human TIMP2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human TIMP2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TIMP2 in the sample.!!Background/Introduction: TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs(kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

NCBI and Uniprot Product Information

• NCBI GI #: 4507511
• NCBI GeneID: 7077
• NCBI Accession #: NP_003246.1
• NCBI GenBank Nucleotide #: NP_003246.1
• UniProt Accession #: P16035
• NCBI Official Full Name: metalloproteinase inhibitor 2
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 2
• NCBI Official Symbol: TIMP2
• NCBI Official Synonym Symbols: DDC8; CSC-21K
• NCBI Protein Information: metalloproteinase inhibitor 2
• UniProt Protein Name: Metalloproteinase inhibitor 2
• UniProt Gene Name: TIMP2
• UniProt Synonym Gene Names: TIMP-2
• UniProt Entry Name: TIMP2_HUMAN

NCBI Description

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Extracellular matrix; Inhibitor; Secreted; Motility/polarity/chemotaxis; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; growth cone; cell soma; extracellular region; basement membrane

Molecular Function: metalloendopeptidase inhibitor activity; integrin binding; protein binding; protease binding; metal ion binding; enzyme activator activity

Biological Process: regulation of Rap protein signal transduction; response to drug; extracellular matrix organization and biogenesis; negative regulation of metalloenzyme activity; central nervous system development; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; extracellular matrix disassembly; positive regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging