TIMP-2 active protein

Human TIMP-2

Cat. Num. AAA695751

• Gene Names: TIMP2; DDC8; CSC-21K; KIAA1731NL
• Reactivity: Species Reactivity: Human
• Purity: Above 95% as determined by SDS-PAGE Analysis.
• Synonyms: TIMP-2; Human TIMP-2; Recombinant Human TIMP-2; TIMP-2 active protein

• Host: E Coli
• Reactivity: Species Reactivity: Human
• Purity/Purification: Above 95% as determined by SDS-PAGE Analysis.
• Sequence: CSCSPVHPQ QAFCNADVV IRAKAVSEK EVDSGNDIY GNPIKRIQY EIKQIKMFK GPEKDIEFI YTAPSSAVC GVSLDVGGK KEYLIAGKA EGDGKMHIT LCDFIVPWD TLSTTQKKS LNHRYQMGC ECKITRCPM IPCYISSPD ECLWMDWVT EKNINGHQA KFFACIKRS DGSCAWYRG AAPPKQEFL DIEDP

• Biological Activity: TIMP-2 activity was measured by its ability to inhibit human MMP-1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP-2 concentration of approximately 0.5 mug/ml, when using an MMP-1
• Preparation and Storage: At -20 degree C

Testing Data

Related Product Information for TIMP-2 active protein

TIMP-2 is an extracellular inhibitor of MMPs including MMP1, 2, 3, 7, 8, 9, 10, 12, 13, 14, 15, 16 and 19. It belongs to the I35 (TIMP) family of irreversible protease inhibitors that function as key modulators of extracellular matrix degradation during tissue development and remodeling. TIMP-2 can also act through an MMP-independent mechanism inhibiting endothelial cell proliferation in vitro and demonstrates anti-angiogenic activities in vivo. Recombinant human TIMP-2 is a 21.8 kDa protein containing 194 amino acid residues.

Product Categories/Family for TIMP-2 active protein

Stem Cell Research; Proliferation and Cell Cycle

NCBI and Uniprot Product Information

• NCBI GI #: 135854
• NCBI GeneID: 7077
• UniProt Accession #: P16035; Q16121; Q93006; Q9UDF7; Q96MC4
• Molecular Weight: 24,399 Da
• NCBI Official Full Name: Metalloproteinase inhibitor 2
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 2
• NCBI Official Symbol: TIMP2
• NCBI Official Synonym Symbols: DDC8; CSC-21K; KIAA1731NL
• NCBI Protein Information: metalloproteinase inhibitor 2; TIMP-2; KIAA1731 N-terminal like protein; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2
• UniProt Protein Name: Metalloproteinase inhibitor 2
• UniProt Gene Name: TIMP2
• UniProt Synonym Gene Names: TIMP-2
• UniProt Entry Name: TIMP2_HUMAN

NCBI Description

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Inhibitor; Cell development/differentiation; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extracellular space; proteinaceous extracellular matrix; growth cone; cell surface; cell soma; extracellular region; basement membrane

Molecular Function: integrin binding; metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding; enzyme activator activity

Biological Process: response to drug; regulation of Rap protein signal transduction; extracellular matrix organization and biogenesis; negative regulation of metalloenzyme activity; central nervous system development; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging