Tissue Inhibitors Of Metalloproteinase 1 Recombinant Protein | TIMP1 recombinant protein

Recombinant Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)

Cat. Num. AAA2029462

• Gene Names: TIMP1; EPA; EPO; HCI; CLGI; TIMP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Tissue Inhibitors Of Metalloproteinase 1; Recombinant Tissue Inhibitors Of Metalloproteinase 1 (TIMP1); TIMP1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; CTCVPPH PQTAFCSSEF VIRAKFVGTS EVNQTTLQRR YEIKMTKMFK GFSALGDAPD TWFVYTPAME SLCGYFHRSE NRSEEFLIAG QLLDEKLYIT TCSFVAPWNS LSSAQRQGFTKTYAAGCGEC SVFPCSSIPC KLQSDTDCLW TDQLLTGS
• Sequence Length: 40

• Applicable Applications for TIMP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 47.3kDa
• Accurate Molecular Mass (KD): 47kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Equus caballus; Equine (Horse)
• Fragment: Cys24~Ser178
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 994731
• NCBI GeneID: 7076
• Molecular Weight: 23,171 Da
• NCBI Official Full Name: tissue inhibitor of metalloproteinase 1, partial
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 1
• NCBI Official Symbol: TIMP1
• NCBI Official Synonym Symbols: EPA; EPO; HCI; CLGI; TIMP
• NCBI Protein Information: metalloproteinase inhibitor 1
• UniProt Protein Name: Metalloproteinase inhibitor 1
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP1
• UniProt Synonym Gene Names: CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1
• UniProt Entry Name: TIMP1_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; growth factor activity; protease binding; metal ion binding; cytokine activity

Biological Process: response to peptide hormone stimulus; platelet activation; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; blood coagulation; negative regulation of apoptosis; aging