Rabbit SP6 Polyclonal Antibody | anti-SP6 antibody

SP6 Antibody (Center)

Cat. Num. AAA9214749

• Gene Names: SP6; EPFN; KLF14; EPIPROFIN
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SP6; Polyclonal Antibody; SP6 Antibody (Center); Transcription factor Sp6; Krueppel-like factor 14; KLF14; anti-SP6 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from the Central region of human SP6.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 196-225
• Sequence Length: 376

• Applicable Applications for anti-SP6 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(SP6 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP6 antibody detected the SP6 protein (arrow).)

Flow Cytometry (FC/FACS)

(SP6 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SP6 antibody

SP6 belongs to a family of transcription factors that
contain 3 classical zinc finger DNA-binding domains consisting of a
zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines
(C2H2 motif). These transcription factors bind to GC-rich sequences
and related GT and CACCC boxes (Scohy et al., 2000 [PubMed
11087666]).

References

Nakamura, T., et al. J. Biol. Chem. 279(1):626-634(2004)
Scohy, S., et al. Genomics 70(1):93-101(2000)

NCBI and Uniprot Product Information

• NCBI GI #: 384871624
• NCBI GeneID: 80320
• NCBI Accession #: NP_001245177.1; NP_954871.1
• NCBI GenBank Nucleotide #: NM_001258248.1
• UniProt Accession #: Q3SY56; B3KXS4
• Molecular Weight: 39840
• NCBI Official Full Name: transcription factor Sp6
• NCBI Official Synonym Full Names: Sp6 transcription factor
• NCBI Official Symbol: SP6
• NCBI Official Synonym Symbols: EPFN; KLF14; EPIPROFIN
• NCBI Protein Information: transcription factor Sp6
• UniProt Protein Name: Transcription factor Sp6
• Protein Family: Transcription factor
• UniProt Gene Name: SP6
• UniProt Synonym Gene Names: KLF14
• UniProt Entry Name: SP6_HUMAN

NCBI Description

SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes (Scohy et al., 2000 [PubMed 11087666]).[supplied by OMIM, Mar 2008]

Uniprot Description

SP6: Promotes cell proliferation. Belongs to the Sp1 C2H2-type zinc-finger protein family.

Protein type: DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of odontogenesis; positive regulation of cell proliferation