MCM7 Polyclonal Antibody | anti-MCM7 antibody

Anti-MCM7 Antibody

Cat. Num. AAA177844

• Gene Names: MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: MCM7; Polyclonal Antibody; Anti-MCM7 Antibody; DNA replication licensing factor MCM7 ; CDABP0042; CDC 47; CDC47; CDC47 homolog; Cdc47; S. cerevisiae; homolog of; DNA replication licensing factor MCM7; Homolog of S. cerevisiae Cdc47; MCM 2; MCM 7; MCM2; formerly; Mcm7; MCM7 minichromosome maintenance deficient 7; MCM7_HUMAN; Minichromosome Maintainence 7; Minichromosome maintainence; Minichromosome maintenance complex component 7; Minichromosome maintenance deficient 7; Minichromosome maintenance protein 7; P1.1 MCM3; P1.1-MCM3; P1CDC47; P85MCM; PNAS 146; PNAS146; minichromosome maintenance complex component 7; anti-MCM7 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 543

• Applicable Applications for anti-MCM7 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml; Immunocytochemistry Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human MCM7 recombinant protein (Position: D526-V719). Human MCM7 shares 94% amino acid (aa) sequence identity with mouse MCM7.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- MCM7 Picoband antibody, MBS177844, Western blottingAll lanes: Anti MCM7 (MBS177844) at 0.5ug/mlWB: Recombinant Human MCM7 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD )

Western Blot (WB)

(Anti- MCM7 Picoband antibody, MBS177844, Western blottingAll lanes: Anti MCM7 (MBS177844) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: NIH3T3 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: JURKAT Whole Cell Lysate at 40ugLane 6: 22RV1 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: PC-12 Whole Cell Lysate at 40ugPredicted bind size: 81KDObserved bind size: 81KD )

Immunohistochemistry (IHC)

(Anti- MCM7 Picoband antibody, MBS177844,IHC(P)IHC(P): Mouse Testis Tissue )

Immunohistochemistry (IHC)

(Anti- MCM7 Picoband antibody, MBS177844,IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti- MCM7 Picoband antibody, MBS177844,IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunocytochemistry (ICC)

(Anti- MCM7 Picoband antibody, MBS177844,ICCICC: A549 Cell )

Related Product Information for anti-MCM7 antibody

Description: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM7(MCM7) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Background: MCM7(Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped to 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. It has been found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.

References

1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Nakatsuru, S., Sudo, K., Nakamura, Y. Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication.Cytogenet. Cell Genet. 68: 226-230, 1995. 3. Petrocca, F., Visone, R., Onelli, M. R., Shah, M. H., Nicoloso, M. S., de Martino, I., Iliopoulos, D., Pilozzi, E., Liu, C.-G., Negrini, M., Cavazzini, L., Volinia, S., Alder, H., Ruco, L. P., Baldassarre, G., Croce, C. M., Vecchione, A. E2F1-regulated microRNAs impair TGF-beta-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell 13: 272-286, 2008.

NCBI and Uniprot Product Information

• NCBI GI #: 518830597
• NCBI GeneID: 4176
• NCBI Accession #: NP_001265524.1
• NCBI GenBank Nucleotide #: NM_001278595.1
• UniProt Accession #: P33993; Q15076; Q96D34; Q96GL1; A4D2A1; A4D2A2; E9PGN9
• Molecular Weight: 60,643 Da
• NCBI Official Full Name: DNA replication licensing factor MCM7 isoform 2
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 7
• NCBI Official Symbol: MCM7
• NCBI Official Synonym Symbols: MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
• NCBI Protein Information: DNA replication licensing factor MCM7
• UniProt Protein Name: DNA replication licensing factor MCM7
• Protein Family: DNA replication licensing factor
• UniProt Gene Name: MCM7
• UniProt Synonym Gene Names: CDC47; MCM2
• UniProt Entry Name: MCM7_HUMAN

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MCM7: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6- MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with the ATR- ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. Belongs to the MCM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.6.4.12; DNA replication

Chromosomal Location of Human Ortholog: 7q21.3-q22.1

Cellular Component: chromatin; cytoplasm; cytosol; MCM complex; membrane; nuclear chromosome, telomeric region; nucleoplasm

Molecular Function: ATP binding; ATP-dependent DNA helicase activity; DNA binding; protein binding; single-stranded DNA binding

Biological Process: cell proliferation; DNA replication; DNA replication initiation; DNA unwinding during replication; G1/S transition of mitotic cell cycle; regulation of phosphorylation; response to DNA damage stimulus; response to drug