Rat DNA Polymerase delta Catalytic Subunit (POLD1) ELISA Kit | POLD1 elisa kit

Rat DNA Polymerase delta Catalytic Subunit (POLD1) ELISA Kit

Cat. Num. AAA9712103

• Reactivity: Rat
• Synonyms: DNA Polymerase delta Catalytic Subunit (POLD1); Rat DNA Polymerase delta Catalytic Subunit (POLD1) ELISA Kit; POLD1; CDC2; POLD; CDC2 homolog; DNA-directed DNA polymerase delta 1; DNA-directed polymerase delta 1; POLD1 elisa kit

• Reactivity: Rat
• Specificity: Rat DNA polymerase delta catalytic subunit (POLD1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat POLD1. No significant cross-reactivity or interference between Rat POLD1 and analogues was observed.
• Sequence Length: 1103

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat DNA polymerase delta catalytic subunit (POLD1) Standard Curve.)

Related Product Information for POLD1 elisa kit

Description: This Rat DNA polymerase delta catalytic subunit (POLD1) ELISA Kit employs a two-site sandwich ELISA to quantitate POLD1 in samples. An antibody specific for POLD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POLD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4. The enzyme is 94% identical to bovine DNA polymerase delta and contains the numerous highly conserved regions previously observed in the bovine and yeast enzymes. The human enzyme also contains 2 putative zinc finger domains in the C-terminal region of the molecule as well as a putative nuclear localization signal at the N-terminal end.WRN functionally interacts with DNA polymerase delta, which is required for DNA replication and DNA repair.

NCBI and Uniprot Product Information

• NCBI GI #: 11067381
• NCBI GeneID: 59294
• NCBI Accession #: NP_067694.1
• NCBI GenBank Nucleotide #: NP_067694.1
• UniProt Accession #: O54747
• NCBI Official Full Name: DNA polymerase delta catalytic subunit
• NCBI Official Synonym Full Names: DNA polymerase delta 1, catalytic subunit
• NCBI Official Symbol: Pold1
• NCBI Protein Information: DNA polymerase delta catalytic subunit
• UniProt Protein Name: DNA polymerase delta catalytic subunit
• Protein Family: DNA polymerase
• UniProt Gene Name: Pold1

NCBI Description

has DNA polymerase and 3'exonuclease activities; plays roles in DNA replication, base excision repair, and other types of DNA repair [RGD, Feb 2006]

Uniprot Description

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites.