TIGIT recombinant protein

Human CellExp TIGIT; human recombinant

Cat. Num. AAA845588

• Gene Names: TIGIT; VSIG9; VSTM3; WUCAM
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: TIGIT; Human CellExp TIGIT; human recombinant; VSIG9; VSTM3; TIGIT recombinant protein

• Host: HEK 293 cells; Source: Human
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS; pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Sequence Length: 244

• Applicable Applications for TIGIT recombinant protein: SDS-PAGE
• Tag Information: This protein is fused with a polyhistidine tag at the C-terminus. The predicted N-terminus is Met 22. DTT-reduced Protein migrates as 18-24 kDa in SDS-PAGE due to glycosylation.
• Endotoxin Level: < 1.0 EU per mug of the protein by the LAL method.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in PBS; pH 7.4. Do not vortex.
• Amino Acid Range: Met 22- Pro 141
• Preparation and Storage: Store at -20 degree C for 12 months.; Centrifuge the vial prior to opening.

Testing Data

Related Product Information for TIGIT recombinant protein

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9); V-set and transmembrane domain-containing protein 3 (VSTM3); which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain; a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

NCBI and Uniprot Product Information

• NCBI GI #: 256600228
• NCBI GeneID: 201633
• NCBI Accession #: NP_776160.2
• NCBI GenBank Nucleotide #: NM_173799.3
• UniProt Accession #: Q495A1; Q495A3; Q5JPD8; Q6MZS2; Q8N877
• Molecular Weight: 15 kDa
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: TIGIT
• NCBI Official Synonym Symbols: VSIG9; VSTM3; WUCAM
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• Protein Family: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: TIGIT
• UniProt Synonym Gene Names: VSIG9; VSTM3

NCBI Description

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]

Uniprot Description

TIGIT: Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: cell surface; cell-cell adherens junction; integral to plasma membrane

Molecular Function: cell adhesion molecule binding; protein binding; protein homodimerization activity; receptor activity; receptor binding

Biological Process: cell recognition; heterophilic cell adhesion; homophilic cell adhesion; negative regulation of interleukin-12 production; negative regulation of T cell activation; positive regulation of interleukin-10 production