TIGIT recombinant protein

TIGIT, 22-141aa, Human, hIgG-His-tag, Baculovirus

Cat. Num. AAA206754

• Gene Names: TIGIT; VSIG9; VSTM3; WUCAM
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TIGIT; 22-141aa; Human; hIgG-His-tag; Baculovirus; T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; WUCAM; TIGIT recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MMTGTIETTG NISAEKGGSI ILQCHLSSTT AQVTQVNWEQ QDQLLAICNA DLGWHISPSF KDRVAPGPGL GLTLQSLTVN DTGEYFCIYH TYPDGTYTGR IFLEVLESSV AEHGARFQIP LEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGKHHHHHH
• Sequence Length: 244

• Applicable Applications for TIGIT recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: hIgG-His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TIGIT recombinant protein

TIGIT, also known as T-cell immunoreceptor with Ig and ITIM domains, is a surface protein containing and immunoglobulin variable domain. This protein is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells. Also, it binds with high affinity to the poliovirus receptor which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Recombinant human TIGIT, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for TIGIT recombinant protein

Immunology

References

Yu X., et al, (2009) Nat. Immunol. 10:48-57.; Godefroy E., et al, (2015) Haematologica 100:1415-1425.

NCBI and Uniprot Product Information

• NCBI GI #: 256600228
• NCBI GeneID: 201633
• NCBI Accession #: NP_776160
• NCBI GenBank Nucleotide #: NM_173799.3
• UniProt Accession #: Q495A1; Q495A3; Q5JPD8; Q6MZS2; Q8N877
• Molecular Weight: 40.0kDa (359aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: TIGIT
• NCBI Official Synonym Symbols: VSIG9; VSTM3; WUCAM
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• Protein Family: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: TIGIT
• UniProt Synonym Gene Names: VSIG9; VSTM3

NCBI Description

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]

Uniprot Description

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.