ALK-5/TGFBR1 recombinant protein

Recombinant Mouse ALK-5/TGFBR1 (C-Fc)

Cat. Num. AAA2569337

• Gene Names: Tgfbr1; ALK5; ESK2; Alk-5; TGFR-1; TbetaRI; AU017191; TbetaR-I
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: ALK-5/TGFBR1; Recombinant Mouse ALK-5/TGFBR1 (C-Fc); AAT5; activin A receptor type II-like kinase; 53kD; ACVRLK4; ALK-5; ALK-5ALK5; LDS1A; LDS2A; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; ALK-5/TGFBR1 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Leu30-Glu125

• Species: Mouse
• Tag: C-Fc
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Mouse TGF-beta Receptor Type-1 is produced by our Mammalian expression system and the target gene encoding Leu30-Glu125 is expressed with a Fc tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

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Related Product Information for ALK-5/TGFBR1 recombinant protein

TGF-beta RI, also called ALK-5, is an approximately 55kDa type I transmembrane serine/threonine receptor kinase. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII. Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3. TGF-beta functions as a tumor suppressor by inhibiting the cell cycle in the G1 phase. Administration of TGF-beta is able to protect against mammary tumor development in transgenic mouse models in vivo. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers, with the majority of colon and gastric cancers being caused by an inactivating mutation of TGF-beta RII. TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells. Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development.

NCBI and Uniprot Product Information

• NCBI GI #: 918410268
• NCBI GeneID: 21812
• NCBI Accession #: NP_001299797.1
• NCBI GenBank Nucleotide #: NM_001312868.1
• UniProt Accession #: Q64729; A2AJN0
• Molecular Weight: 55,790 Da
• NCBI Official Full Name: TGF-beta receptor type-1 isoform 2
• NCBI Official Synonym Full Names: transforming growth factor, beta receptor I
• NCBI Official Symbol: Tgfbr1
• NCBI Official Synonym Symbols: ALK5; ESK2; Alk-5; TGFR-1; TbetaRI; AU017191; TbetaR-I
• NCBI Protein Information: TGF-beta receptor type-1
• UniProt Protein Name: TGF-beta receptor type-1
• UniProt Gene Name: Tgfbr1
• UniProt Synonym Gene Names: TGFR-1; TGF-beta receptor type I; TbetaR-I

NCBI Description

This gene encodes a member of the transforming growth factor beta (TGF-beta) receptor family of proteins. These proteins comprise one component of the TGF-beta signaling pathway, which transduces extracellular signals into gene expression changes to regulate a wide range of cellular responses, including proliferation, migration, differentiation and apoptosis. Homozygous knockout mice for this gene exhibit impaired angiogenesis and embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation ().