Western Blot (WB)
(Western Blot: Sample: Human Lung lysate; Primary Ab: 2ug/ml Mouse Anti-Human GS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Mouse anti-Human Gelsolin Monoclonal Antibody | anti-GS antibody
Monoclonal Antibody to Gelsolin (GS)
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-AMAAQHGMD DDGTGQKQIW RIEGSNKVPV DPATYGQFYG GDSYIILYNY RHGGRQGQII YNWQGAQSTQ DEVAASAILT AQLDEELGGT PVQSRVVQGK EPAHLMSLFG GKPMIIYKGG TSR
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human Lung lysate; Primary Ab: 2ug/ml Mouse Anti-Human GS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Lung lysate; Primary Ab: 2ug/ml Mouse Anti-Human GS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant Gelsolin, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant Gelsolin, Human.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation.
Protein type: Actin-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9q33.2
Cellular Component: actin cap; actin cytoskeleton; blood microparticle; cortical actin cytoskeleton; cytoplasm; cytosol; extracellular exosome; extracellular region; extracellular space; ficolin-1-rich granule lumen; focal adhesion; lamellipodium; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; podosome; ruffle; sarcoplasm; secretory granule lumen
Molecular Function: actin binding; actin filament binding; calcium ion binding; myosin II binding; protein binding
Biological Process: actin filament capping; actin filament polymerization; actin filament reorganization; actin filament severing; actin nucleation; aging; amyloid fibril formation; barbed-end actin filament capping; cellular protein metabolic process; cellular response to cadmium ion; extracellular matrix disassembly; negative regulation of virion penetration into host cell; neutrophil degranulation; oligodendrocyte development; phagocytosis, engulfment; phosphoinositide-mediated signaling; positive regulation of actin nucleation; positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; positive regulation of gene expression; positive regulation of keratinocyte apoptotic process; positive regulation of protein processing in phagocytic vesicle; protein destabilization; regulation of establishment of T cell polarity; regulation of plasma membrane raft polarization; regulation of podosome assembly; regulation of receptor clustering; response to ethanol; response to folic acid; sequestering of actin monomers; striated muscle atrophy; tissue regeneration
Disease: Amyloidosis, Finnish Type
Research Articles on GS
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Product Notes
The GS gsn (Catalog #AAA2025766) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Gelsolin (GS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Gelsolin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-5ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GS gsn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -AMAAQHGMD DDGTGQKQIW RIEGSNKVPV DPATYGQFYG GDSYIILYNY RHGGRQGQII YNWQGAQSTQ DEVAASAILT AQLDEELGGT PVQSRVVQGK EPAHLMSLFG GKPMIIYKGG TSR. It is sometimes possible for the material contained within the vial of "Gelsolin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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