Bovine transforming growth factor, beta 1 ELISA Kit | TGFB1 elisa kit

Bovine transforming growth factor beta1, TGF-beta1 ELISA Kit

Cat. Num. AAA702883

• Reactivity: Bovine
• Synonyms: transforming growth factor; beta 1; Bovine transforming growth factor beta1; TGF-beta1 ELISA Kit; CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide; TGFB1 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bovine TGFB1. No significant cross-reactivity or interference between Bovine TGFB1 and analogues was observed.
• Sequence Length: 390

• Samples: Serum, plasma, cell culture supernates
• Detection Range: 6.25 ng/ml-400 ng/ml
• Sensitivity: 1.56 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGFB1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TGF-beta1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TGF-beta1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TGF-beta1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 260655603
• NCBI GeneID: 282089
• NCBI Accession #: NP_001159540.1
• NCBI GenBank Nucleotide #: NM_001166068.1
• UniProt Accession #: P18341; A5A3B8
• Molecular Weight: 44,409 Da
• NCBI Official Full Name: transforming growth factor beta-1
• NCBI Official Symbol: TGFB1
• NCBI Protein Information: transforming growth factor beta-1; TGF-beta-1; transforming growth factor-beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease)
• UniProt Protein Name: Transforming growth factor beta-1
• Protein Family: Transforming growth factor
• UniProt Gene Name: TGFB1
• UniProt Synonym Gene Names: TGF-beta-1; LAP
• UniProt Entry Name: TGFB1_BOVIN

Uniprot Description

Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts