Bovine Transforming Growth Factor Beta 1 (TGFb1) RTU ELISA Kit | TGFb1 rtu elisa kit

Bovine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

Cat. Num. AAA4502805

• Reactivity: Bovine
• Synonyms: Transforming Growth Factor Beta 1 (TGFb1); Bovine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide; TGFb1 rtu elisa kit

• Reactivity: Bovine
• Sequence Length: 390

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 10.7pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGFb1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGFb1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TGFb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 260655603
• NCBI GeneID: 282089
• NCBI Accession #: NP_001159540.1
• NCBI GenBank Nucleotide #: NM_001166068.1
• UniProt Accession #: P18341; A5A3B8
• Molecular Weight: 44,409 Da
• NCBI Official Full Name: transforming growth factor beta-1
• NCBI Official Synonym Full Names: transforming growth factor beta 1
• NCBI Official Symbol: TGFB1
• NCBI Protein Information: transforming growth factor beta-1
• UniProt Protein Name: Transforming growth factor beta-1
• Protein Family: Transforming growth factor
• UniProt Gene Name: TGFB1
• UniProt Synonym Gene Names: TGF-beta-1; LAP

Uniprot Description

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types ().